TY - JOUR

T1 - Hydrolysis of triglycerides in milk to provide fatty acids as precursors in the formation of deep eutectic solvent for extraction of polycyclic aromatic hydrocarbons

AU - Shakirova, Firuza

AU - Shishov, Andrey

AU - Bulatov, Andrey

N1 - Publisher Copyright: © 2021 Elsevier B.V.

PY - 2022/1/15

Y1 - 2022/1/15

N2 - A microextraction pretreatment for powdered milk analysis, relying on the formation of a natural deep eutectic solvent is proposed. It relies on the in situ hydrolysis of milk fats (triglycerides) which yields fatty acids as precursors in the formation of the natural deep eutectic solvent. As a proof-of-concept, the innovation was applied to the determination of thirteen polycyclic aromatic hydrocarbons in powdered milk samples by HPLC with fluorometric detection. The alkaline hydrolysis of milk triglycerides minimized sample matrices interference through removal of proteins and fats, and led to formation of natural deep eutectic solvent precursors (fatty acids) directly from the sample components. Addition of only one precursor (terpenoid) was then required. Menthol and thymol (natural terpenoids) were investigated as deep eutectic solvent precursors for micro extraction of polycyclic aromatic hydrocarbons. Under the selected experimental conditions, limits of detection were estimated within the 0.002-0.09 mu g kg-1 range. The innovation provided satisfactory (70-91%) extraction of hydrophobic analytes from complex powdered milk matrices containing hydrophobic components (triglycerides) without the need for hazardous organic solvents. The RSD values were

AB - A microextraction pretreatment for powdered milk analysis, relying on the formation of a natural deep eutectic solvent is proposed. It relies on the in situ hydrolysis of milk fats (triglycerides) which yields fatty acids as precursors in the formation of the natural deep eutectic solvent. As a proof-of-concept, the innovation was applied to the determination of thirteen polycyclic aromatic hydrocarbons in powdered milk samples by HPLC with fluorometric detection. The alkaline hydrolysis of milk triglycerides minimized sample matrices interference through removal of proteins and fats, and led to formation of natural deep eutectic solvent precursors (fatty acids) directly from the sample components. Addition of only one precursor (terpenoid) was then required. Menthol and thymol (natural terpenoids) were investigated as deep eutectic solvent precursors for micro extraction of polycyclic aromatic hydrocarbons. Under the selected experimental conditions, limits of detection were estimated within the 0.002-0.09 mu g kg-1 range. The innovation provided satisfactory (70-91%) extraction of hydrophobic analytes from complex powdered milk matrices containing hydrophobic components (triglycerides) without the need for hazardous organic solvents. The RSD values were

KW - Microextraction

KW - Natural deep eutectic solvent

KW - Polycyclic aromatic hydrocarbons

KW - Powdered milk

KW - Triglycerides hydrolysis

KW - MICROEXTRACTION

KW - CHROMATOGRAPHY

KW - PESTICIDES

UR - http://www.scopus.com/inward/record.url?scp=85117364194&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/8265d5d9-3b8a-33c8-aa9e-cf934c9781bc/

U2 - 10.1016/j.talanta.2021.122968

DO - 10.1016/j.talanta.2021.122968

M3 - Article

AN - SCOPUS:85117364194

VL - 237

JO - Talanta

JF - Talanta

SN - 0039-9140

M1 - 122968

ER -