Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Humoral responses of congenital immunity in the starfish Asterias rubens. / Kudryavtsev, I. V.; D'yachkov, I. S.; Kazakov, A. A.; Kanaikin, D. P.; Kharazova, A. D.; Polevshchikov, A. V.
в: Journal of Evolutionary Biochemistry and Physiology, Том 41, № 1, 01.01.2005, стр. 26-33.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Humoral responses of congenital immunity in the starfish Asterias rubens
AU - Kudryavtsev, I. V.
AU - D'yachkov, I. S.
AU - Kazakov, A. A.
AU - Kanaikin, D. P.
AU - Kharazova, A. D.
AU - Polevshchikov, A. V.
PY - 2005/1/1
Y1 - 2005/1/1
N2 - The work presents analysis of changes of humoral protective factors in the starfish Asterias rubens in response to injection of human erythrocytes (HE). The total protein concentration and the titers of hemagglutinins and hemolysins in starfish coelomic fluid, as well as the time of human hemoglobin elimination from circulation were estimated for 6-144 h of the experiment. The hemagglutinin titer was determined in hemagglutination reactions, the hemolysin titer-in hemolysis reaction. Time of human hemoglobin elimination from coelomic fluid was determined in a color enzymatic reaction. The starfish coelomic fluid was revealed to contain soluble factors that are able to interact with antigen- antibody complexes of mammals and have an opsonizing activity. It is established that injection of HE does not change the total protein concentration per 1 ml coelomic fluid, but affects dynamics of changes of the hemagglutinins titer. Time of hemoglobin elimination from circulation does not exceed 24 h. Humoral factors of coelomic fluid of the starfish Asterias rubens play an auxiliary role in congenital immunity reactions.
AB - The work presents analysis of changes of humoral protective factors in the starfish Asterias rubens in response to injection of human erythrocytes (HE). The total protein concentration and the titers of hemagglutinins and hemolysins in starfish coelomic fluid, as well as the time of human hemoglobin elimination from circulation were estimated for 6-144 h of the experiment. The hemagglutinin titer was determined in hemagglutination reactions, the hemolysin titer-in hemolysis reaction. Time of human hemoglobin elimination from coelomic fluid was determined in a color enzymatic reaction. The starfish coelomic fluid was revealed to contain soluble factors that are able to interact with antigen- antibody complexes of mammals and have an opsonizing activity. It is established that injection of HE does not change the total protein concentration per 1 ml coelomic fluid, but affects dynamics of changes of the hemagglutinins titer. Time of hemoglobin elimination from circulation does not exceed 24 h. Humoral factors of coelomic fluid of the starfish Asterias rubens play an auxiliary role in congenital immunity reactions.
UR - http://www.scopus.com/inward/record.url?scp=17844404866&partnerID=8YFLogxK
U2 - 10.1007/s10893-005-0031-5
DO - 10.1007/s10893-005-0031-5
M3 - Article
AN - SCOPUS:17844404866
VL - 41
SP - 26
EP - 33
JO - Journal of Evolutionary Biochemistry and Physiology
JF - Journal of Evolutionary Biochemistry and Physiology
SN - 0022-0930
IS - 1
ER -
ID: 41027986