Standard

Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. / Hillman, C; Fontana, BD; Amstislavskaya, TG; Gorbunova, MA; Altenhofen, S; Barthelson, K; Bastos, LM; Borba, JV; Bonan, CD; Brennan, CH; Farias-Cea, A; Cooper, A; Corcoran, J; Dondossola, ER; Martinez-Duran, LM; Gallas-Lopes, M; Galstyan, DS; Garcia, EO; Gerken, E; Hindges, R; Kenney, JW; Kleshchev, MA; Kolesnikova, TO; Leggieri, A; Khatsko, SL; Lardelli, M; Lodetti, G; Lombardelli, G; Luchiari, AC; Portela, SM; Medan, V; Moutinho, LM; Nekhoroshev, EV; Petersen, BD; Petrunich-Rutherford, ML; Piato, A; Porfiri, M; Read, E; Resmim, CM; Rico, EP; Rosemberg, DB; de Abreu, MS; Salazar, CA; Stahlhofer-Buss, T; Teixeira, JR; Valentim, AM; Zhdanov, AV; Iturriaga-Vásquez, P; Wang, X; Wong, RY; Kalueff, AV; Parker, MO.

в: Lab Animal, Том 54, № 6, 2025.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Hillman, C, Fontana, BD, Amstislavskaya, TG, Gorbunova, MA, Altenhofen, S, Barthelson, K, Bastos, LM, Borba, JV, Bonan, CD, Brennan, CH, Farias-Cea, A, Cooper, A, Corcoran, J, Dondossola, ER, Martinez-Duran, LM, Gallas-Lopes, M, Galstyan, DS, Garcia, EO, Gerken, E, Hindges, R, Kenney, JW, Kleshchev, MA, Kolesnikova, TO, Leggieri, A, Khatsko, SL, Lardelli, M, Lodetti, G, Lombardelli, G, Luchiari, AC, Portela, SM, Medan, V, Moutinho, LM, Nekhoroshev, EV, Petersen, BD, Petrunich-Rutherford, ML, Piato, A, Porfiri, M, Read, E, Resmim, CM, Rico, EP, Rosemberg, DB, de Abreu, MS, Salazar, CA, Stahlhofer-Buss, T, Teixeira, JR, Valentim, AM, Zhdanov, AV, Iturriaga-Vásquez, P, Wang, X, Wong, RY, Kalueff, AV & Parker, MO 2025, 'Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study', Lab Animal, Том. 54, № 6. https://doi.org/10.1038/s41684-025-01548-x

APA

Hillman, C., Fontana, BD., Amstislavskaya, TG., Gorbunova, MA., Altenhofen, S., Barthelson, K., Bastos, LM., Borba, JV., Bonan, CD., Brennan, CH., Farias-Cea, A., Cooper, A., Corcoran, J., Dondossola, ER., Martinez-Duran, LM., Gallas-Lopes, M., Galstyan, DS., Garcia, EO., Gerken, E., ... Parker, MO. (2025). Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. Lab Animal, 54(6). https://doi.org/10.1038/s41684-025-01548-x

Vancouver

Hillman C, Fontana BD, Amstislavskaya TG, Gorbunova MA, Altenhofen S, Barthelson K и пр. Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. Lab Animal. 2025;54(6). https://doi.org/10.1038/s41684-025-01548-x

Author

Hillman, C ; Fontana, BD ; Amstislavskaya, TG ; Gorbunova, MA ; Altenhofen, S ; Barthelson, K ; Bastos, LM ; Borba, JV ; Bonan, CD ; Brennan, CH ; Farias-Cea, A ; Cooper, A ; Corcoran, J ; Dondossola, ER ; Martinez-Duran, LM ; Gallas-Lopes, M ; Galstyan, DS ; Garcia, EO ; Gerken, E ; Hindges, R ; Kenney, JW ; Kleshchev, MA ; Kolesnikova, TO ; Leggieri, A ; Khatsko, SL ; Lardelli, M ; Lodetti, G ; Lombardelli, G ; Luchiari, AC ; Portela, SM ; Medan, V ; Moutinho, LM ; Nekhoroshev, EV ; Petersen, BD ; Petrunich-Rutherford, ML ; Piato, A ; Porfiri, M ; Read, E ; Resmim, CM ; Rico, EP ; Rosemberg, DB ; de Abreu, MS ; Salazar, CA ; Stahlhofer-Buss, T ; Teixeira, JR ; Valentim, AM ; Zhdanov, AV ; Iturriaga-Vásquez, P ; Wang, X ; Wong, RY ; Kalueff, AV ; Parker, MO. / Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. в: Lab Animal. 2025 ; Том 54, № 6.

BibTeX

@article{2e6500d9e86a419a8b88d1a51baa36cd,
title = "Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study",
abstract = "The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.",
keywords = "REPRODUCIBILITY, STRESS, ADULT, ETHANOL, MODEL, WATER, REPLICATION, COMORBIDITY, LOCOMOTOR, ROTIFERS",
author = "C Hillman and BD Fontana and TG Amstislavskaya and MA Gorbunova and S Altenhofen and K Barthelson and LM Bastos and JV Borba and CD Bonan and CH Brennan and A Farias-Cea and A Cooper and J Corcoran and ER Dondossola and LM Martinez-Duran and M Gallas-Lopes and DS Galstyan and EO Garcia and E Gerken and R Hindges and JW Kenney and MA Kleshchev and TO Kolesnikova and A Leggieri and SL Khatsko and M Lardelli and G Lodetti and G Lombardelli and AC Luchiari and SM Portela and V Medan and LM Moutinho and EV Nekhoroshev and BD Petersen and ML Petrunich-Rutherford and A Piato and M Porfiri and E Read and CM Resmim and EP Rico and DB Rosemberg and {de Abreu}, MS and CA Salazar and T Stahlhofer-Buss and JR Teixeira and AM Valentim and AV Zhdanov and P Iturriaga-V{\'a}squez and X Wang and RY Wong and AV Kalueff and MO Parker",
note = "Times Cited in Web of Science Core Collection: 7 Total Times Cited: 8 Cited Reference Count: 109",
year = "2025",
doi = "10.1038/s41684-025-01548-x",
language = "English",
volume = "54",
journal = "Lab Animal",
issn = "0093-7355",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study

AU - Hillman, C

AU - Fontana, BD

AU - Amstislavskaya, TG

AU - Gorbunova, MA

AU - Altenhofen, S

AU - Barthelson, K

AU - Bastos, LM

AU - Borba, JV

AU - Bonan, CD

AU - Brennan, CH

AU - Farias-Cea, A

AU - Cooper, A

AU - Corcoran, J

AU - Dondossola, ER

AU - Martinez-Duran, LM

AU - Gallas-Lopes, M

AU - Galstyan, DS

AU - Garcia, EO

AU - Gerken, E

AU - Hindges, R

AU - Kenney, JW

AU - Kleshchev, MA

AU - Kolesnikova, TO

AU - Leggieri, A

AU - Khatsko, SL

AU - Lardelli, M

AU - Lodetti, G

AU - Lombardelli, G

AU - Luchiari, AC

AU - Portela, SM

AU - Medan, V

AU - Moutinho, LM

AU - Nekhoroshev, EV

AU - Petersen, BD

AU - Petrunich-Rutherford, ML

AU - Piato, A

AU - Porfiri, M

AU - Read, E

AU - Resmim, CM

AU - Rico, EP

AU - Rosemberg, DB

AU - de Abreu, MS

AU - Salazar, CA

AU - Stahlhofer-Buss, T

AU - Teixeira, JR

AU - Valentim, AM

AU - Zhdanov, AV

AU - Iturriaga-Vásquez, P

AU - Wang, X

AU - Wong, RY

AU - Kalueff, AV

AU - Parker, MO

N1 - Times Cited in Web of Science Core Collection: 7 Total Times Cited: 8 Cited Reference Count: 109

PY - 2025

Y1 - 2025

N2 - The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.

AB - The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.

KW - REPRODUCIBILITY

KW - STRESS

KW - ADULT

KW - ETHANOL

KW - MODEL

KW - WATER

KW - REPLICATION

KW - COMORBIDITY

KW - LOCOMOTOR

KW - ROTIFERS

U2 - 10.1038/s41684-025-01548-x

DO - 10.1038/s41684-025-01548-x

M3 - Article

VL - 54

JO - Lab Animal

JF - Lab Animal

SN - 0093-7355

IS - 6

ER -

ID: 147948934