Standard

Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system. / Shiriaeva, Anna A.; Kuznedelov, Konstanin; Fedorov, Ivan; Musharova, Olga; Khvostikov, Timofey; Tsoy, Yuliya; Kurilovich, Elena; Smith, Gerald R.; Semenova, Ekaterina; Severinov, Konstantin.

в: Science advances, Том 8, № 47, eabn8650, 11.2022.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Shiriaeva, AA, Kuznedelov, K, Fedorov, I, Musharova, O, Khvostikov, T, Tsoy, Y, Kurilovich, E, Smith, GR, Semenova, E & Severinov, K 2022, 'Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system', Science advances, Том. 8, № 47, eabn8650. https://doi.org/10.1126/sciadv.abn8650

APA

Shiriaeva, A. A., Kuznedelov, K., Fedorov, I., Musharova, O., Khvostikov, T., Tsoy, Y., Kurilovich, E., Smith, G. R., Semenova, E., & Severinov, K. (2022). Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system. Science advances, 8(47), [eabn8650]. https://doi.org/10.1126/sciadv.abn8650

Vancouver

Shiriaeva AA, Kuznedelov K, Fedorov I, Musharova O, Khvostikov T, Tsoy Y и пр. Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system. Science advances. 2022 Нояб.;8(47). eabn8650. https://doi.org/10.1126/sciadv.abn8650

Author

Shiriaeva, Anna A. ; Kuznedelov, Konstanin ; Fedorov, Ivan ; Musharova, Olga ; Khvostikov, Timofey ; Tsoy, Yuliya ; Kurilovich, Elena ; Smith, Gerald R. ; Semenova, Ekaterina ; Severinov, Konstantin. / Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system. в: Science advances. 2022 ; Том 8, № 47.

BibTeX

@article{9d560c3172e34c2f8f2f6febfe9e4b22,
title = "Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system",
abstract = "CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic acids. In Escherichia coli, immunity is acquired upon integration of 33-bp spacers into CRISPR arrays. DNA targets complementary to spacers get degraded and serve as a source of new spacers during a process called primed adaptation. Precursors of such spacers, prespacers, are ~33-bp double-stranded DNA fragments with a ~4-nt 3′ overhang. The mechanism of prespacer generation is not clear. Here, we use FragSeq and biochemical approaches to determine enzymes involved in generation of defined prespacer ends. We demonstrate that RecJ is the main exonuclease trimming 5′ ends of prespacer precursors, although its activity can be partially substituted by ExoVII. The RecBCD complex allows single strand–specific RecJ to process double-stranded regions flanking prespacers. Our results reveal intricate functional interactions of genome maintenance proteins with CRISPR interference and adaptation machineries during generation of prespacers capable of integration into CRISPR arrays.",
author = "Shiriaeva, {Anna A.} and Konstanin Kuznedelov and Ivan Fedorov and Olga Musharova and Timofey Khvostikov and Yuliya Tsoy and Elena Kurilovich and Smith, {Gerald R.} and Ekaterina Semenova and Konstantin Severinov",
note = "Publisher Copyright: Copyright {\textcopyright} 2022 The Authors, some rights reserved;",
year = "2022",
month = nov,
doi = "10.1126/sciadv.abn8650",
language = "English",
volume = "8",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "47",

}

RIS

TY - JOUR

T1 - Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system

AU - Shiriaeva, Anna A.

AU - Kuznedelov, Konstanin

AU - Fedorov, Ivan

AU - Musharova, Olga

AU - Khvostikov, Timofey

AU - Tsoy, Yuliya

AU - Kurilovich, Elena

AU - Smith, Gerald R.

AU - Semenova, Ekaterina

AU - Severinov, Konstantin

N1 - Publisher Copyright: Copyright © 2022 The Authors, some rights reserved;

PY - 2022/11

Y1 - 2022/11

N2 - CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic acids. In Escherichia coli, immunity is acquired upon integration of 33-bp spacers into CRISPR arrays. DNA targets complementary to spacers get degraded and serve as a source of new spacers during a process called primed adaptation. Precursors of such spacers, prespacers, are ~33-bp double-stranded DNA fragments with a ~4-nt 3′ overhang. The mechanism of prespacer generation is not clear. Here, we use FragSeq and biochemical approaches to determine enzymes involved in generation of defined prespacer ends. We demonstrate that RecJ is the main exonuclease trimming 5′ ends of prespacer precursors, although its activity can be partially substituted by ExoVII. The RecBCD complex allows single strand–specific RecJ to process double-stranded regions flanking prespacers. Our results reveal intricate functional interactions of genome maintenance proteins with CRISPR interference and adaptation machineries during generation of prespacers capable of integration into CRISPR arrays.

AB - CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic acids. In Escherichia coli, immunity is acquired upon integration of 33-bp spacers into CRISPR arrays. DNA targets complementary to spacers get degraded and serve as a source of new spacers during a process called primed adaptation. Precursors of such spacers, prespacers, are ~33-bp double-stranded DNA fragments with a ~4-nt 3′ overhang. The mechanism of prespacer generation is not clear. Here, we use FragSeq and biochemical approaches to determine enzymes involved in generation of defined prespacer ends. We demonstrate that RecJ is the main exonuclease trimming 5′ ends of prespacer precursors, although its activity can be partially substituted by ExoVII. The RecBCD complex allows single strand–specific RecJ to process double-stranded regions flanking prespacers. Our results reveal intricate functional interactions of genome maintenance proteins with CRISPR interference and adaptation machineries during generation of prespacers capable of integration into CRISPR arrays.

UR - http://www.scopus.com/inward/record.url?scp=85142940341&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/4e62b218-c4dd-35e2-95bb-cf534ffa9136/

U2 - 10.1126/sciadv.abn8650

DO - 10.1126/sciadv.abn8650

M3 - Article

VL - 8

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 47

M1 - eabn8650

ER -

ID: 100595491