TY - JOUR

T1 - Host association and intracellularity evolved multiple times independently in the Rickettsiales

AU - Castelli, M.

AU - Nardi, T.

AU - Gammuto, L.

AU - Bellinzona, G.

AU - Sabaneyeva, E.

AU - Potekhin, A.

AU - Serra, V.

AU - Petroni, G.

AU - Sassera, D.

N1 - Export Date: 21 March 2024 Адрес для корреспонденции: Sassera, D.; Department of Biology and Biotechnology, Italy; эл. почта: davide.sassera@unipv.it Адрес для корреспонденции: Petroni, G.; Department of Biology, Italy; эл. почта: giulio.petroni@unipi.it Сведения о финансировании: European Commission, EC, PE00000007, RSF 20-14-00220 Сведения о финансировании: Human Frontier Science Program, HFSP, RGY-0075 Сведения о финансировании: Ministero dell’Istruzione, dell’Università e della Ricerca, MIUR Сведения о финансировании: Università degli Studi di Pavia, 872767 Сведения о финансировании: Università di Pisa, UniPi Текст о финансировании 1: This project was supported by the Human Frontier Science Program (HFSP) Young Investigator Program grant RGY-0075 to D.S., by the Italian Ministry of Education, University and Research (MIUR): Dipartimenti di Eccellenza Programme (2018–2022) Department of Biology and Biotechnology ‘L. Spallanzani’ University of Pavia to D.S., by the European Community’s H2020 Programme H2020-MSCA-RISE 2019 under grant agreement n° 872767 to G.P., and by EU funding within the NextGenerationEU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases (Project no. PE00000007, INF-ACT) to M.C. and D.S. Genomic characterisation of Trichorickettsia and Megaira venefica was performed partly with support of RSF 20-14-00220 to A.P. The University of Pisa is acknowledged for providing visiting scholarships to E.S. and A.P. The authors would like to thank Venkata Mahesh Nitla for support in culturing Plagiopyla frontata IBS-3, Sascha Krenek for DNA preparation of the Paramecium biaurelia strain US_Bl 11III1, Umberto Postiglione for assistance in Nanopore assembly, Laura Quattrini and Marco Fagioli for assistance in genome closing by PCR. Gernot Gloeckner and Marco Groth are gratefully acknowledged for sharing genome sequencing data on Reticulomyxa filosa and its associated Rickettsiales bacterium.

PY - 2024/2/6

Y1 - 2024/2/6

N2 - The order Rickettsiales (Alphaproteobacteria) encompasses multiple diverse lineages of host-associated bacteria, including pathogens, reproductive manipulators, and mutualists. Here, in order to understand how intracellularity and host association originated in this order, and whether they are ancestral or convergently evolved characteristics, we built a large and phylogenetically-balanced dataset that includes de novo sequenced genomes and a selection of published genomic and metagenomic assemblies. We perform detailed functional reconstructions that clearly indicates “late” and parallel evolution of obligate host-association in different Rickettsiales lineages. According to the depicted scenario, multiple independent horizontal acquisitions of transporters led to the progressive loss of biosynthesis of nucleotides, amino acids and other metabolites, producing distinct conditions of host-dependence. Each clade experienced a different pattern of evolution of the ancestral arsenal of interaction apparatuses, including development of specialised effectors involved in the lineage-specific mechanisms of host cell adhesion and/or invasion. © The Author(s) 2024.

AB - The order Rickettsiales (Alphaproteobacteria) encompasses multiple diverse lineages of host-associated bacteria, including pathogens, reproductive manipulators, and mutualists. Here, in order to understand how intracellularity and host association originated in this order, and whether they are ancestral or convergently evolved characteristics, we built a large and phylogenetically-balanced dataset that includes de novo sequenced genomes and a selection of published genomic and metagenomic assemblies. We perform detailed functional reconstructions that clearly indicates “late” and parallel evolution of obligate host-association in different Rickettsiales lineages. According to the depicted scenario, multiple independent horizontal acquisitions of transporters led to the progressive loss of biosynthesis of nucleotides, amino acids and other metabolites, producing distinct conditions of host-dependence. Each clade experienced a different pattern of evolution of the ancestral arsenal of interaction apparatuses, including development of specialised effectors involved in the lineage-specific mechanisms of host cell adhesion and/or invasion. © The Author(s) 2024.

KW - bacterial disease

KW - cell component

KW - detection method

KW - parallel evolution

KW - Alphaproteobacteria

KW - cytoplasm

KW - genetics

KW - metagenome

KW - molecular evolution

KW - phylogeny

KW - Rickettsiales

KW - Cytoplasm

KW - Evolution, Molecular

KW - Metagenome

KW - Phylogeny

KW - Rickettsiales/genetics

KW - Alphaproteobacteria/genetics

UR - https://www.mendeley.com/catalogue/a561d3fe-51e5-38ba-854a-cfc716cc52e9/

U2 - 10.1038/s41467-024-45351-7

DO - 10.1038/s41467-024-45351-7

M3 - статья

C2 - 38321113

VL - 15

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -