Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide

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Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide. / Nikolaev, K. G.; Ermakov, S. S.; Ermolenko, Yu E.; Navolotskaya, D. V.; Offenhäusser, A.; Mourzina, Yu G.

в: Journal of Analytical Chemistry, Том 76, № 4, 04.2021, стр. 510-517.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{7d1ab0c2ab8a49099e1da318f113954e,

title = "Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide",

abstract = "Abstract: We studied methods of the in situ synthesis of gold nanostructures on the electrode surface and of the directed electrochemical synthesis of nanodendrites to create biosensors based on horseradish peroxidase. The effect of nanostructuring of the transducer surface and its subsequent modification on the electroanalytical characteristics of sensors for the determination of hydrogen peroxide was studied. The use of these methods can significantly improve the performance characteristics of the biosensors, such as the sensitivity coefficient, limit of detection, and stability in the range of micromolar and submicromolar concentrations of hydrogen peroxide. The electrodes modified by the in situ synthesis of nanostructures with subsequent treatment with Meerwein{\textquoteright}s reagent, as well as ultramicroelectrodes prepared by directional electrochemical synthesis, show low limits of detection for hydrogen peroxide, 0.2 and 0.08 µM, and high sensitivity coefficients, 0.71 and 0.84 A M–1 cm–2, respectively.",

keywords = "electrochemical biosensor, enzyme, gold nanostructures, hydrogen peroxide, modified electrode, peroxidase, ELECTRODES, SENSOR, GOLD NANOSTRUCTURES, ELECTRICAL CONNECTION, GLUCOSE, DIRECT ELECTROCHEMISTRY, REDOX CENTERS, SYSTEMS, SURFACES",

author = "Nikolaev, {K. G.} and Ermakov, {S. S.} and Ermolenko, {Yu E.} and Navolotskaya, {D. V.} and A. Offenh{\"a}usser and Mourzina, {Yu G.}",

year = "2021",

month = apr,

doi = "10.1134/s1061934821040080",

language = "English",

volume = "76",

pages = "510--517",

journal = "Journal of Analytical Chemistry",

issn = "1061-9348",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "4",

}

RIS

TY - JOUR

T1 - Horseradish Peroxidase-Based Biosensors with Different Nanotransducers for the Determination of Hydrogen Peroxide

AU - Nikolaev, K. G.

AU - Ermakov, S. S.

AU - Ermolenko, Yu E.

AU - Navolotskaya, D. V.

AU - Offenhäusser, A.

AU - Mourzina, Yu G.

PY - 2021/4

Y1 - 2021/4

N2 - Abstract: We studied methods of the in situ synthesis of gold nanostructures on the electrode surface and of the directed electrochemical synthesis of nanodendrites to create biosensors based on horseradish peroxidase. The effect of nanostructuring of the transducer surface and its subsequent modification on the electroanalytical characteristics of sensors for the determination of hydrogen peroxide was studied. The use of these methods can significantly improve the performance characteristics of the biosensors, such as the sensitivity coefficient, limit of detection, and stability in the range of micromolar and submicromolar concentrations of hydrogen peroxide. The electrodes modified by the in situ synthesis of nanostructures with subsequent treatment with Meerwein’s reagent, as well as ultramicroelectrodes prepared by directional electrochemical synthesis, show low limits of detection for hydrogen peroxide, 0.2 and 0.08 µM, and high sensitivity coefficients, 0.71 and 0.84 A M–1 cm–2, respectively.

AB - Abstract: We studied methods of the in situ synthesis of gold nanostructures on the electrode surface and of the directed electrochemical synthesis of nanodendrites to create biosensors based on horseradish peroxidase. The effect of nanostructuring of the transducer surface and its subsequent modification on the electroanalytical characteristics of sensors for the determination of hydrogen peroxide was studied. The use of these methods can significantly improve the performance characteristics of the biosensors, such as the sensitivity coefficient, limit of detection, and stability in the range of micromolar and submicromolar concentrations of hydrogen peroxide. The electrodes modified by the in situ synthesis of nanostructures with subsequent treatment with Meerwein’s reagent, as well as ultramicroelectrodes prepared by directional electrochemical synthesis, show low limits of detection for hydrogen peroxide, 0.2 and 0.08 µM, and high sensitivity coefficients, 0.71 and 0.84 A M–1 cm–2, respectively.

KW - electrochemical biosensor

KW - enzyme

KW - gold nanostructures

KW - hydrogen peroxide

KW - modified electrode

KW - peroxidase

KW - ELECTRODES

KW - SENSOR

KW - GOLD NANOSTRUCTURES

KW - ELECTRICAL CONNECTION

KW - GLUCOSE

KW - DIRECT ELECTROCHEMISTRY

KW - REDOX CENTERS

KW - SYSTEMS

KW - SURFACES

UR - http://www.scopus.com/inward/record.url?scp=85104382635&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/7e8ff258-dcc5-3bea-baa4-5bf1cc00b93c/

U2 - 10.1134/s1061934821040080

DO - 10.1134/s1061934821040080

M3 - Article

AN - SCOPUS:85104382635

VL - 76

SP - 510

EP - 517

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 4

ER -

ID: 76606097