Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
hnRNP-K Targets Open Chromatin in Mouse Embryonic Stem Cells in Concert with Multiple Regulators. / Bakhmet, Evgeny I.; Nazarov, Igor B.; Gazizova, Adel R.; Vorobyeva, Nadezhda E.; Kuzmin, Andrey A.; Gordeev, Mikhail N.; Sinenko, Sergey A.; Aksenov, Nikolai D.; Artamonova, Tatyana O.; Khodorkovskii, Mikhail A.; Alenina, Natalia; Onichtchouk, Daria; Wu, Guangming; Schöler, Hans R.; Tomilin, Alexey N.
в: Stem Cells, Том 37, № 8, 08.2019, стр. 1018-1029.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - hnRNP-K Targets Open Chromatin in Mouse Embryonic Stem Cells in Concert with Multiple Regulators
AU - Bakhmet, Evgeny I.
AU - Nazarov, Igor B.
AU - Gazizova, Adel R.
AU - Vorobyeva, Nadezhda E.
AU - Kuzmin, Andrey A.
AU - Gordeev, Mikhail N.
AU - Sinenko, Sergey A.
AU - Aksenov, Nikolai D.
AU - Artamonova, Tatyana O.
AU - Khodorkovskii, Mikhail A.
AU - Alenina, Natalia
AU - Onichtchouk, Daria
AU - Wu, Guangming
AU - Schöler, Hans R.
AU - Tomilin, Alexey N.
PY - 2019/8
Y1 - 2019/8
N2 - The transcription factor Oct4 plays a key regulatory role in the induction and maintenance of cellular pluripotency. In this article, we show that ubiquitous and multifunctional poly(C) DNA/RNA-binding protein hnRNP-K occupies Oct4 (Pou5f1) enhancers in embryonic stem cells (ESCs) but is dispensable for the initiation, maintenance, and downregulation of Oct4 gene expression. Nevertheless, hnRNP-K has an essential cell-autonomous function in ESCs to maintain their proliferation and viability. To better understand mechanisms of hnRNP-K action in ESCs, we have performed ChIP-seq analysis of genome-wide binding of hnRNP-K and identified several thousands of hnRNP-K target sites that are frequently co-occupied by pluripotency-related and common factors (Oct4, TATA-box binding protein, Sox2, Nanog, Otx2, etc.), as well as active histone marks. Furthermore, hnRNP-K localizes exclusively within open chromatin, implying its role in the onset and/or maintenance of this chromatin state. Stem Cells 2019;37:1018–1029.
AB - The transcription factor Oct4 plays a key regulatory role in the induction and maintenance of cellular pluripotency. In this article, we show that ubiquitous and multifunctional poly(C) DNA/RNA-binding protein hnRNP-K occupies Oct4 (Pou5f1) enhancers in embryonic stem cells (ESCs) but is dispensable for the initiation, maintenance, and downregulation of Oct4 gene expression. Nevertheless, hnRNP-K has an essential cell-autonomous function in ESCs to maintain their proliferation and viability. To better understand mechanisms of hnRNP-K action in ESCs, we have performed ChIP-seq analysis of genome-wide binding of hnRNP-K and identified several thousands of hnRNP-K target sites that are frequently co-occupied by pluripotency-related and common factors (Oct4, TATA-box binding protein, Sox2, Nanog, Otx2, etc.), as well as active histone marks. Furthermore, hnRNP-K localizes exclusively within open chromatin, implying its role in the onset and/or maintenance of this chromatin state. Stem Cells 2019;37:1018–1029.
KW - Chromatin
KW - Histone code
KW - hnRNP-K
KW - Oct4
KW - Pluripotent stem cells
KW - Pou5f1
UR - http://www.scopus.com/inward/record.url?scp=85068190098&partnerID=8YFLogxK
U2 - 10.1002/stem.3025
DO - 10.1002/stem.3025
M3 - Article
C2 - 31021473
AN - SCOPUS:85068190098
VL - 37
SP - 1018
EP - 1029
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 8
ER -
ID: 50500760