Standard

High-performance membrane chromatography of small molecules. / Podgomik, A.; Barut, M.; Jancar, J.; Strancar, A.; Tennikova, T.

в: Analytical Chemistry, Том 71, № 15, 01.08.1999, стр. 2986-2991.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Podgomik, A, Barut, M, Jancar, J, Strancar, A & Tennikova, T 1999, 'High-performance membrane chromatography of small molecules', Analytical Chemistry, Том. 71, № 15, стр. 2986-2991. https://doi.org/10.1021/ac981350v

APA

Podgomik, A., Barut, M., Jancar, J., Strancar, A., & Tennikova, T. (1999). High-performance membrane chromatography of small molecules. Analytical Chemistry, 71(15), 2986-2991. https://doi.org/10.1021/ac981350v

Vancouver

Podgomik A, Barut M, Jancar J, Strancar A, Tennikova T. High-performance membrane chromatography of small molecules. Analytical Chemistry. 1999 Авг. 1;71(15):2986-2991. https://doi.org/10.1021/ac981350v

Author

Podgomik, A. ; Barut, M. ; Jancar, J. ; Strancar, A. ; Tennikova, T. / High-performance membrane chromatography of small molecules. в: Analytical Chemistry. 1999 ; Том 71, № 15. стр. 2986-2991.

BibTeX

@article{f28ad43b7a03409eba7af48635bd12af,
title = "High-performance membrane chromatography of small molecules",
abstract = "High-performance membrane chromatography (HPMC) proved to be a very efficient method for fast protein separations. Recently, it was shown to be applicable also for the isocratic chromatography of plasmid DNA conformations. However, no study about the separation of small molecules has been performed until now. In this work, we investigated the possibility of gradient and isocratic HPMC of small molecules with Convective Interaction Media disks of different chemistries and tried to explain the mechanism that enables their separation. We demonstrated that it is possible to achieve efficient separations of oligonucleotides and peptides in the ion-exchange mode as well as the separation of small hydrophobic molecules in the reversed-phase mode. It was shown that similar peak resolution can be provided in both gradient and isocratic modes.",
author = "A. Podgomik and M. Barut and J. Jancar and A. Strancar and T. Tennikova",
year = "1999",
month = aug,
day = "1",
doi = "10.1021/ac981350v",
language = "English",
volume = "71",
pages = "2986--2991",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "15",

}

RIS

TY - JOUR

T1 - High-performance membrane chromatography of small molecules

AU - Podgomik, A.

AU - Barut, M.

AU - Jancar, J.

AU - Strancar, A.

AU - Tennikova, T.

PY - 1999/8/1

Y1 - 1999/8/1

N2 - High-performance membrane chromatography (HPMC) proved to be a very efficient method for fast protein separations. Recently, it was shown to be applicable also for the isocratic chromatography of plasmid DNA conformations. However, no study about the separation of small molecules has been performed until now. In this work, we investigated the possibility of gradient and isocratic HPMC of small molecules with Convective Interaction Media disks of different chemistries and tried to explain the mechanism that enables their separation. We demonstrated that it is possible to achieve efficient separations of oligonucleotides and peptides in the ion-exchange mode as well as the separation of small hydrophobic molecules in the reversed-phase mode. It was shown that similar peak resolution can be provided in both gradient and isocratic modes.

AB - High-performance membrane chromatography (HPMC) proved to be a very efficient method for fast protein separations. Recently, it was shown to be applicable also for the isocratic chromatography of plasmid DNA conformations. However, no study about the separation of small molecules has been performed until now. In this work, we investigated the possibility of gradient and isocratic HPMC of small molecules with Convective Interaction Media disks of different chemistries and tried to explain the mechanism that enables their separation. We demonstrated that it is possible to achieve efficient separations of oligonucleotides and peptides in the ion-exchange mode as well as the separation of small hydrophobic molecules in the reversed-phase mode. It was shown that similar peak resolution can be provided in both gradient and isocratic modes.

UR - http://www.scopus.com/inward/record.url?scp=0032781051&partnerID=8YFLogxK

U2 - 10.1021/ac981350v

DO - 10.1021/ac981350v

M3 - Article

AN - SCOPUS:0032781051

VL - 71

SP - 2986

EP - 2991

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 15

ER -

ID: 87865703