We carried out highly rapid laser-assisted deposition of conductive nickel microstructures on the surface of oxide glass from a deep eutectic solvent (DES). The implementation of deep eutectic solvents may significantly simplify the experimental procedure and drastically increase the deposition rate by more than 150 times in opposite to those observed for laser deposition from the aqueous solutions. It was shown that these structures exhibit promising electrocatalytic performance toward enzyme-free dopamine and acetaminophen sensing, including good sensitivity, low limit of detection and broad linear ranges.