TY - JOUR

T1 - High performance flow injection analysis of recombinant Protein G

AU - Hagedorn, Jörg

AU - Kasper, Cornelia

AU - Freitag, Ruth

AU - Tennikova, Tatiana

PY - 1999/3/26

Y1 - 1999/3/26

N2 - Chromatographic discs were investigated for their potential to substitute for the hitherto used cartridges in heterogeneous flow injection analysis. Originally designed for fast high performance liquid chromatography (HPLC) of biopolymers, the discs combine reliability with speed and resolution. This together with their price and their long-standing time made them attractive for use in flow injection analysis. The base material of the discs is a glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) co-polymer. The epoxy groups inherent to this base structure can be used for immobilization purposes. In this first demonstration, antibodies were immobilized and the resulting affinity discs used for the fast analysis (<5 min) of protein G from cell lysate of recombinant Escherichia coli. A linear calibration curve over several orders of magnitude as well as excellent reproducibility and correlation with data produced by conventional protein assay were obtained. Copyright (C) 1999 Elsevier Science B.V.

AB - Chromatographic discs were investigated for their potential to substitute for the hitherto used cartridges in heterogeneous flow injection analysis. Originally designed for fast high performance liquid chromatography (HPLC) of biopolymers, the discs combine reliability with speed and resolution. This together with their price and their long-standing time made them attractive for use in flow injection analysis. The base material of the discs is a glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) co-polymer. The epoxy groups inherent to this base structure can be used for immobilization purposes. In this first demonstration, antibodies were immobilized and the resulting affinity discs used for the fast analysis (<5 min) of protein G from cell lysate of recombinant Escherichia coli. A linear calibration curve over several orders of magnitude as well as excellent reproducibility and correlation with data produced by conventional protein assay were obtained. Copyright (C) 1999 Elsevier Science B.V.

KW - Flow injection analysis

KW - Membrane chromatography

KW - Recombinant Protein G

UR - http://www.scopus.com/inward/record.url?scp=0033053995&partnerID=8YFLogxK

U2 - 10.1016/S0168-1656(98)00181-3

DO - 10.1016/S0168-1656(98)00181-3

M3 - Article

C2 - 10201110

AN - SCOPUS:0033053995

VL - 69

SP - 1

EP - 7

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1

ER -