TY - JOUR

T1 - High genetic diversity of amoebae belonging to the genus Mayorella (Amoebozoa, Discosea, Dermamoebida) in natural habitats

AU - Глотова, Анна Андреевна

AU - Бондаренко, Наталья Ивановна

AU - Смирнов, Алексей Валерьевич

PY - 2018/1/15

Y1 - 2018/1/15

N2 - Amoebae of the genus Mayorella are widespread in marine, freshwater and soil habitats. These amoebae have relatively lownumber of morphological characters allowing species differentiation, so the number of valid species remains rather small. Representativesof the genus Mayorella are hard to maintain in culture, the very most of them are polyphagous and carnivorous. To live and multiplythey require a variety of food objects, including other protists. Thus they are difficult objects for molecular studies. Only two sequences ofMayorella were available until recently in GenBank. For the present study we isolated eight strains of Mayorella from different locationsworldwide, documented them with the light microscopy and obtained complete or partial sequences of their 18S rRNA gene. Results showthat all members of the genus Mayorella form a robust clade within the order Dermamoebida (Amoebozoa: Discosea). The vast majorityof our strains probably represent new species; this evidences that diversity of the genus Mayorella in natural habitats is high and that thisgenus is rather species-rich, comprising no less than 11 species. Our results show that nowadays sequence data are necessary for reliableidentification of Mayorella species.

AB - Amoebae of the genus Mayorella are widespread in marine, freshwater and soil habitats. These amoebae have relatively lownumber of morphological characters allowing species differentiation, so the number of valid species remains rather small. Representativesof the genus Mayorella are hard to maintain in culture, the very most of them are polyphagous and carnivorous. To live and multiplythey require a variety of food objects, including other protists. Thus they are difficult objects for molecular studies. Only two sequences ofMayorella were available until recently in GenBank. For the present study we isolated eight strains of Mayorella from different locationsworldwide, documented them with the light microscopy and obtained complete or partial sequences of their 18S rRNA gene. Results showthat all members of the genus Mayorella form a robust clade within the order Dermamoebida (Amoebozoa: Discosea). The vast majorityof our strains probably represent new species; this evidences that diversity of the genus Mayorella in natural habitats is high and that thisgenus is rather species-rich, comprising no less than 11 species. Our results show that nowadays sequence data are necessary for reliableidentification of Mayorella species.

KW - Amoebozoa

KW - Diversity

KW - Mayorella

KW - Phylogeny

KW - Systematics

UR - http://www.scopus.com/inward/record.url?scp=85056670814&partnerID=8YFLogxK

U2 - 10.4467/16890027AP.18.002.8396

DO - 10.4467/16890027AP.18.002.8396

M3 - Article

VL - 57

SP - 29

EP - 42

JO - Acta Protozoologica

JF - Acta Protozoologica

SN - 0065-1583

IS - 1

ER -