TY - CHAP

T1 - Glycation of Plant Proteins under Environmental Stress - Methodological Approaches, Potential Mechanisms and Biological Role

AU - Bilova, Tatiana

AU - Greifenhagen, Uta

AU - Paudel, Gagan

AU - Lukasheva, Elena

AU - Brauch, Dominic

AU - Osmolovskaya, Natalia

AU - Tarakhovskaya, Elena

AU - Balcke, Gerd Ulrich

AU - Tissier, Alain

AU - Vogt, Thomas

AU - Milkowski, Carsten

AU - Birkemeyer, Claudia

AU - Wessjohann, Ludger

AU - Frolov, Andrej

PY - 2016

Y1 - 2016

N2 - Environmental stress is one of the major factors reducing crop productivity. Due to the oncoming climate changes, the effects of drought and high light on plants play an increasing role in modern agriculture. These changes are accompanied with a progressing contamination of soils with heavy metals. Independent of their nature, environmental alterations result in development of oxidative stress, i.e. increase of reactive oxygen species (ROS) contents, and metabolic adjustment, i.e. accumulation of soluble primary metabolites (amino acids and sugars). However, a simultaneous increase of ROS and sugar concentrations ultimately results in protein glycation, i.e. non-enzymatic interaction of reducing sugars or their degradation products (alpha-dicarbonyls) with proteins. The eventually resulting advanced glycation end-products (AGEs) are known to be toxic and pro-inflammatory in mammals. Recently, their presence was unambiguously demonstrated in vivo in stressed Arabidopsis thaliana plants. Currently, information on protein targets, modification sites therein, mediators and mechanisms of plant glycation are being intensively studied. In this chapter, we comprehensively review the methodological approaches for plant glycation research and discuss potential mechanisms of AGE formation under stress conditions. On the basis of these patterns and additional in vitro experiments, the pathways and mechanisms of plant glycation can be proposed.

AB - Environmental stress is one of the major factors reducing crop productivity. Due to the oncoming climate changes, the effects of drought and high light on plants play an increasing role in modern agriculture. These changes are accompanied with a progressing contamination of soils with heavy metals. Independent of their nature, environmental alterations result in development of oxidative stress, i.e. increase of reactive oxygen species (ROS) contents, and metabolic adjustment, i.e. accumulation of soluble primary metabolites (amino acids and sugars). However, a simultaneous increase of ROS and sugar concentrations ultimately results in protein glycation, i.e. non-enzymatic interaction of reducing sugars or their degradation products (alpha-dicarbonyls) with proteins. The eventually resulting advanced glycation end-products (AGEs) are known to be toxic and pro-inflammatory in mammals. Recently, their presence was unambiguously demonstrated in vivo in stressed Arabidopsis thaliana plants. Currently, information on protein targets, modification sites therein, mediators and mechanisms of plant glycation are being intensively studied. In this chapter, we comprehensively review the methodological approaches for plant glycation research and discuss potential mechanisms of AGE formation under stress conditions. On the basis of these patterns and additional in vitro experiments, the pathways and mechanisms of plant glycation can be proposed.

KW - Advanced glycation end-products (AGEs)

KW - Ageing

KW - Environmental stress

KW - Glycation

KW - Proteomics

U2 - 10.5772/61860

DO - 10.5772/61860

M3 - глава/раздел

SN - 978-953-51-2250-0

SP - 295

EP - 316

BT - ABIOTIC AND BIOTIC STRESS IN PLANTS - RECENT ADVANCES AND FUTURE PERSPECTIVES

A2 - Shanker, AK

A2 - Shanker, C

PB - InTech

ER -