Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique. / Galaktionov, N.K.; Podgornaya, O.I.; Strelkov, P.P.; Galaktionov, K. V.
в: Parasitology Research, Том 115, № 12, 2016, стр. 4587–4593.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique
AU - Galaktionov, N.K.
AU - Podgornaya, O.I.
AU - Strelkov, P.P.
AU - Galaktionov, K. V.
PY - 2016
Y1 - 2016
N2 - The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability
AB - The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability
KW - AFLP
KW - H. elongata
KW - parthenitae
KW - Clonal diversity
KW - Monomiracidial infection
U2 - 10.1007/s00436-016-5249-1
DO - 10.1007/s00436-016-5249-1
M3 - Article
VL - 115
SP - 4587
EP - 4593
JO - Parasitology Research
JF - Parasitology Research
SN - 0932-0113
IS - 12
ER -
ID: 7593648