Standard

Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1). / Herlemann, D. P.R.; Geissinger, O.; Ikeda-Ohtsubo, W.; Kunin, V.; Sun, H.; Lapidus, A.; Hugenholtz, P.; Brune, A.

в: Applied and Environmental Microbiology, Том 75, № 9, 05.2009, стр. 2841-2849.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Herlemann, DPR, Geissinger, O, Ikeda-Ohtsubo, W, Kunin, V, Sun, H, Lapidus, A, Hugenholtz, P & Brune, A 2009, 'Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1)', Applied and Environmental Microbiology, Том. 75, № 9, стр. 2841-2849. https://doi.org/10.1128/AEM.02698-08

APA

Herlemann, D. P. R., Geissinger, O., Ikeda-Ohtsubo, W., Kunin, V., Sun, H., Lapidus, A., Hugenholtz, P., & Brune, A. (2009). Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1). Applied and Environmental Microbiology, 75(9), 2841-2849. https://doi.org/10.1128/AEM.02698-08

Vancouver

Herlemann DPR, Geissinger O, Ikeda-Ohtsubo W, Kunin V, Sun H, Lapidus A и пр. Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1). Applied and Environmental Microbiology. 2009 Май;75(9):2841-2849. https://doi.org/10.1128/AEM.02698-08

Author

Herlemann, D. P.R. ; Geissinger, O. ; Ikeda-Ohtsubo, W. ; Kunin, V. ; Sun, H. ; Lapidus, A. ; Hugenholtz, P. ; Brune, A. / Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1). в: Applied and Environmental Microbiology. 2009 ; Том 75, № 9. стр. 2841-2849.

BibTeX

@article{cdfe7918837640aeaa1c5b473633c2fb,
title = "Genomic analysis of {"}Elusimicrobium minutum{"} the first cultivated representative of the phylum {"}Elusimicrobia{"} (formerly termite group 1)",
abstract = "Organisms of the candidate phylum termite group 1 (TG1) are regularly encountered in termite hindguts but are present also in many other habitats. Here, we report the complete genome sequence (1.64 Mbp) of {"}Elusimicrobium minutum{"} strain Pei191 T, the first cultured representative of the TG1 phylum. We reconstructed the metabolism of this strictly anaerobic bacterium isolated from a beetle larva gut, and we discuss the findings in light of physiological data. E. minutum has all genes required for uptake and fermentation of sugars via the Embden-Meyerhof pathway, including several hydrogenases, and an unusual peptide degradation pathway comprising transamination reactions and leading to the formation of alanine, which is excreted in substantial amounts. The presence of genes encoding lipopolysaccharide biosynthesis and the presence of a pathway for peptidoglycan formation are consistent with ultrastructural evidence of a gram-negative cell envelope. Even though electron micrographs showed no cell appendages, the genome encodes many genes putatively involved in pilus assembly. We assigned some to a type II secretion system, but the function of 60 pilE-like genes remains unknown. Numerous genes with hypothetical functions, e.g., polyketide synthesis, nonribosomal peptide synthesis, antibiotic transport, and oxygen stress protection, indicate the presence of hitherto undiscovered physiological traits. Comparative analysis of 22 concatenated single-copy marker genes corroborated the status of {"}Elusimicrobia{"} (formerly TG1) as a separate phylum in the bacterial domain, which was so far based only on 16S rRNA sequence analysis.",
author = "Herlemann, {D. P.R.} and O. Geissinger and W. Ikeda-Ohtsubo and V. Kunin and H. Sun and A. Lapidus and P. Hugenholtz and A. Brune",
year = "2009",
month = may,
doi = "10.1128/AEM.02698-08",
language = "English",
volume = "75",
pages = "2841--2849",
journal = "Applied Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "9",

}

RIS

TY - JOUR

T1 - Genomic analysis of "Elusimicrobium minutum" the first cultivated representative of the phylum "Elusimicrobia" (formerly termite group 1)

AU - Herlemann, D. P.R.

AU - Geissinger, O.

AU - Ikeda-Ohtsubo, W.

AU - Kunin, V.

AU - Sun, H.

AU - Lapidus, A.

AU - Hugenholtz, P.

AU - Brune, A.

PY - 2009/5

Y1 - 2009/5

N2 - Organisms of the candidate phylum termite group 1 (TG1) are regularly encountered in termite hindguts but are present also in many other habitats. Here, we report the complete genome sequence (1.64 Mbp) of "Elusimicrobium minutum" strain Pei191 T, the first cultured representative of the TG1 phylum. We reconstructed the metabolism of this strictly anaerobic bacterium isolated from a beetle larva gut, and we discuss the findings in light of physiological data. E. minutum has all genes required for uptake and fermentation of sugars via the Embden-Meyerhof pathway, including several hydrogenases, and an unusual peptide degradation pathway comprising transamination reactions and leading to the formation of alanine, which is excreted in substantial amounts. The presence of genes encoding lipopolysaccharide biosynthesis and the presence of a pathway for peptidoglycan formation are consistent with ultrastructural evidence of a gram-negative cell envelope. Even though electron micrographs showed no cell appendages, the genome encodes many genes putatively involved in pilus assembly. We assigned some to a type II secretion system, but the function of 60 pilE-like genes remains unknown. Numerous genes with hypothetical functions, e.g., polyketide synthesis, nonribosomal peptide synthesis, antibiotic transport, and oxygen stress protection, indicate the presence of hitherto undiscovered physiological traits. Comparative analysis of 22 concatenated single-copy marker genes corroborated the status of "Elusimicrobia" (formerly TG1) as a separate phylum in the bacterial domain, which was so far based only on 16S rRNA sequence analysis.

AB - Organisms of the candidate phylum termite group 1 (TG1) are regularly encountered in termite hindguts but are present also in many other habitats. Here, we report the complete genome sequence (1.64 Mbp) of "Elusimicrobium minutum" strain Pei191 T, the first cultured representative of the TG1 phylum. We reconstructed the metabolism of this strictly anaerobic bacterium isolated from a beetle larva gut, and we discuss the findings in light of physiological data. E. minutum has all genes required for uptake and fermentation of sugars via the Embden-Meyerhof pathway, including several hydrogenases, and an unusual peptide degradation pathway comprising transamination reactions and leading to the formation of alanine, which is excreted in substantial amounts. The presence of genes encoding lipopolysaccharide biosynthesis and the presence of a pathway for peptidoglycan formation are consistent with ultrastructural evidence of a gram-negative cell envelope. Even though electron micrographs showed no cell appendages, the genome encodes many genes putatively involved in pilus assembly. We assigned some to a type II secretion system, but the function of 60 pilE-like genes remains unknown. Numerous genes with hypothetical functions, e.g., polyketide synthesis, nonribosomal peptide synthesis, antibiotic transport, and oxygen stress protection, indicate the presence of hitherto undiscovered physiological traits. Comparative analysis of 22 concatenated single-copy marker genes corroborated the status of "Elusimicrobia" (formerly TG1) as a separate phylum in the bacterial domain, which was so far based only on 16S rRNA sequence analysis.

UR - http://www.scopus.com/inward/record.url?scp=65549153499&partnerID=8YFLogxK

U2 - 10.1128/AEM.02698-08

DO - 10.1128/AEM.02698-08

M3 - Article

C2 - 19270133

AN - SCOPUS:65549153499

VL - 75

SP - 2841

EP - 2849

JO - Applied Microbiology

JF - Applied Microbiology

SN - 0099-2240

IS - 9

ER -

ID: 90034032