TY - JOUR

T1 - Genome composition analysis of reassortant influenza viruses used in seasonal and pandemic live attenuated influenza vaccine1

AU - Kiseleva, Irina V.

AU - Voeten, J. T.M.

AU - Teley, L. C.P.

AU - Larionova, N. V.

AU - Dubrovina, I. A.

AU - Berdygulova, Zh A.

AU - Bazhenova, E. A.

AU - Van Den Bosch, H.

AU - Heldens, J. G.M.

AU - Rudenkoa, L. G.

N1 - Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2011/12

Y1 - 2011/12

N2 - The cold-adapted, temperature sensitive and attenuated influenza master donor viruses A/Lenin- grad/134/17/57 (H2N2) and B/USSR/60/69 were used to generate vaccine viruses to be included in live attenuated influenza vaccine. These vaccine viruses typically are 6 : 2 reassortant viruses containing the gene segments of the surface antigens haemagglutinin and neuraminidase of current wild type influenza A and influenza B viruses with the gene segments encoding the internal viral proteins, conferring the cold-adapted, temperature sensitive and attenuated phenotype, being inherited from the master donor viruses. The 6 : 2 reassortant viruses are selected from co-infections between master donor virus and wild type viruses that the- oretically may yield as many as 256 combinations of gene segments and thus 256 enetically different viruses. As the time to generate and isolate vaccine viruses is limited and because only 6 : 2 reassortant viruses are allowed as vaccine viruses, sboth selection and creening needs to be both rapid and unambiguous. The screen- ing of reassortant viruses by RT-PCRs using master donor virus and wild type virus specific primer sets is described to select both influenza A and influenza B 6 : 2 reassortant viruses to be used in seasonal and pan- demic live attenuated vaccine, unambigously.

AB - The cold-adapted, temperature sensitive and attenuated influenza master donor viruses A/Lenin- grad/134/17/57 (H2N2) and B/USSR/60/69 were used to generate vaccine viruses to be included in live attenuated influenza vaccine. These vaccine viruses typically are 6 : 2 reassortant viruses containing the gene segments of the surface antigens haemagglutinin and neuraminidase of current wild type influenza A and influenza B viruses with the gene segments encoding the internal viral proteins, conferring the cold-adapted, temperature sensitive and attenuated phenotype, being inherited from the master donor viruses. The 6 : 2 reassortant viruses are selected from co-infections between master donor virus and wild type viruses that the- oretically may yield as many as 256 combinations of gene segments and thus 256 enetically different viruses. As the time to generate and isolate vaccine viruses is limited and because only 6 : 2 reassortant viruses are allowed as vaccine viruses, sboth selection and creening needs to be both rapid and unambiguous. The screen- ing of reassortant viruses by RT-PCRs using master donor virus and wild type virus specific primer sets is described to select both influenza A and influenza B 6 : 2 reassortant viruses to be used in seasonal and pan- demic live attenuated vaccine, unambigously.

KW - Genome composition analysis

KW - Influenza virus

KW - Live influenza vaccine

KW - Reassortants

UR - http://www.scopus.com/inward/record.url?scp=84856472501&partnerID=8YFLogxK

U2 - 10.3103/S0891416811040045

DO - 10.3103/S0891416811040045

M3 - Article

AN - SCOPUS:84856472501

VL - 26

SP - 174

EP - 185

JO - Molecular Genetics, Microbiology and Virology

JF - Molecular Genetics, Microbiology and Virology

SN - 0891-4168

IS - 4

ER -