Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Functionalization of polyacrylamide for nanotrapping positively charged biomolecules. / Davydova, Nadejda; Xavier, Rodriguez; Blazquez, Carlos ; Gomez, Andres ; Perevyazko, Igor ; Guasch, Judith ; Sergeev, Vladimir ; Laukhina, Elena ; Ratera, Imma ; Veciana, Jaume .
в: RSC Advances, Том 9, № 27, 19.05.2019, стр. 15402-15409.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Functionalization of polyacrylamide for nanotrapping positively charged biomolecules
AU - Davydova, Nadejda
AU - Xavier, Rodriguez
AU - Blazquez, Carlos
AU - Gomez, Andres
AU - Perevyazko, Igor
AU - Guasch, Judith
AU - Sergeev, Vladimir
AU - Laukhina, Elena
AU - Ratera, Imma
AU - Veciana, Jaume
PY - 2019/5/19
Y1 - 2019/5/19
N2 - Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases. This article demonstrates that using free radical copolymerization, polyacrylamide can be successfully functionalized with specific synthons for nanotrapping positively charged molecules, such as numerous proteins, through electrostatic interactions due to their negative charge. Specifically, two functional random copolymers, acrylamide/acrylic acid (1) and acrylamide/acrylic acid/N-(pyridin-4-yl-methyl)acrylamide (2), whose negative net charges differ in their water solutions, were synthetized and their ability to trap positively charged proteins was studied using myoglobin as a proof-of-concept example. In aqueous solutions, copolymer 1, whose net charge for a 100 chain fragment (Q pH 6/M) is -1.323 × 10 -3, interacted with myoglobin forming a stable monodisperse nanosuspension. In contrast, copolymer 2, whose value of Q pH 6/M equals -0.361 × 10 -3, was not able to form stable particles with myoglobin. Nevertheless, thin films of both copolymers were grown using a dewetting process, which exhibited nanoscale cavities capable of trapping different amounts of myoglobin, as demonstrated by bimodal AFM imaging. The simple procedures used to build protein traps make this engineering approach promising for the development of new materials for biomedical applications where trapping biomolecules is required.
AB - Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases. This article demonstrates that using free radical copolymerization, polyacrylamide can be successfully functionalized with specific synthons for nanotrapping positively charged molecules, such as numerous proteins, through electrostatic interactions due to their negative charge. Specifically, two functional random copolymers, acrylamide/acrylic acid (1) and acrylamide/acrylic acid/N-(pyridin-4-yl-methyl)acrylamide (2), whose negative net charges differ in their water solutions, were synthetized and their ability to trap positively charged proteins was studied using myoglobin as a proof-of-concept example. In aqueous solutions, copolymer 1, whose net charge for a 100 chain fragment (Q pH 6/M) is -1.323 × 10 -3, interacted with myoglobin forming a stable monodisperse nanosuspension. In contrast, copolymer 2, whose value of Q pH 6/M equals -0.361 × 10 -3, was not able to form stable particles with myoglobin. Nevertheless, thin films of both copolymers were grown using a dewetting process, which exhibited nanoscale cavities capable of trapping different amounts of myoglobin, as demonstrated by bimodal AFM imaging. The simple procedures used to build protein traps make this engineering approach promising for the development of new materials for biomedical applications where trapping biomolecules is required.
KW - DNA
KW - FILMS
KW - MARKER
KW - MYOGLOBIN
KW - OPTICAL MANIPULATION
KW - PARTICLE
KW - TRAP
UR - http://www.scopus.com/inward/record.url?scp=85065997785&partnerID=8YFLogxK
U2 - 10.1039/c8ra07764a
DO - 10.1039/c8ra07764a
M3 - Article
VL - 9
SP - 15402
EP - 15409
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 27
ER -
ID: 49358376