TY - JOUR

T1 - Full Transcriptome Profiling of the Liver of Fat-, Fructose- and Cholesterol-Fed C57Black/6J Mice

AU - Apryatin, S. A.

AU - Trusov, N. V.

AU - Gorbachev, A. J.

AU - Naumov, V. A.

AU - Mzhelskaya, K. V.

AU - Balakina, A. S.

AU - Gmoshinski, I. V.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Abstract: C57black/6J female mice were fed an isocaloric diet with excess fat, fructose, cholesterol, or combinations of them for 62 days. In their liver, the differential expression of 30 003 genes was studied by full-chip transcriptional profiling on an Agilent One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling, version 6.8, and the relative levels of mRNA for the genes Gpr19, P2ry12, Gnb1, Csf2rb, Gm38450, Pgm3, Prom2, and Adgrv1 were also evaluated by real-time RT-PCR. To determine the metabolic pathways (KEGG), which are the targets of the applied dietary influences, we analyzed the transcriptome data by bioinformatics methods in the R programming environment. We revealed a specific effect of experimental diets on the metabolic pathways of JAK-STAT and MAPK kinases, VEGF and TOR signaling pathways, and a family of transcription factors PPAR. It reflects the changes occurring in the liver in intracellular signaling, inflammation and angiogenesis. The consequences of such changes may be the development of insulin resistance and fatty hepatosis. For the first time, we revealed experimentally a specific effect of all kinds of diets on metabolic pathways of amino acid metabolism of aspartate, glutamate, alanine, proline, histidine, and arginine. Using real-time RT-PCR, we analyzed the samples derived from the groups of animals that received excess fat with fructose or cholesterol. We detected the coordinated changes in expression levels of the Gpr19, Adgrv1, and Csf2rb genes, reflecting the integrated shifts in the cytokine production profiles and the neurohormonal regulation of metabolic processes.

AB - Abstract: C57black/6J female mice were fed an isocaloric diet with excess fat, fructose, cholesterol, or combinations of them for 62 days. In their liver, the differential expression of 30 003 genes was studied by full-chip transcriptional profiling on an Agilent One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling, version 6.8, and the relative levels of mRNA for the genes Gpr19, P2ry12, Gnb1, Csf2rb, Gm38450, Pgm3, Prom2, and Adgrv1 were also evaluated by real-time RT-PCR. To determine the metabolic pathways (KEGG), which are the targets of the applied dietary influences, we analyzed the transcriptome data by bioinformatics methods in the R programming environment. We revealed a specific effect of experimental diets on the metabolic pathways of JAK-STAT and MAPK kinases, VEGF and TOR signaling pathways, and a family of transcription factors PPAR. It reflects the changes occurring in the liver in intracellular signaling, inflammation and angiogenesis. The consequences of such changes may be the development of insulin resistance and fatty hepatosis. For the first time, we revealed experimentally a specific effect of all kinds of diets on metabolic pathways of amino acid metabolism of aspartate, glutamate, alanine, proline, histidine, and arginine. Using real-time RT-PCR, we analyzed the samples derived from the groups of animals that received excess fat with fructose or cholesterol. We detected the coordinated changes in expression levels of the Gpr19, Adgrv1, and Csf2rb genes, reflecting the integrated shifts in the cytokine production profiles and the neurohormonal regulation of metabolic processes.

KW - dyslipidemia

KW - in vivo model

KW - liver

KW - mice

KW - RT-PCR

KW - transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85066502672&partnerID=8YFLogxK

U2 - 10.1134/S1022795419040021

DO - 10.1134/S1022795419040021

M3 - Article

AN - SCOPUS:85066502672

VL - 55

SP - 399

EP - 410

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 4

ER -