New criteria for evaluating the limits of the mass overloading of chromatographic systems, based on the retention parameters of analytes depending on an amount, peak broadening factors, and the distortion of peak shapes, are compared. The criterion based on the dependence of the retention indices on the masses of analytes in chromatographic zones suggests a qualitative assessment of the effect of the column temperature on the overloading limits. Such an effect observed in gas chromatography has no analogues in the other separation methods. The two other criteria give overloading limits coinciding satisfactorily with each other for both nonpolar and polar analytes. It is confirmed that the anomalies in the temperature dependences of the retention indices of some polar compounds at nonpolar phases (transformation of monotonic functions into dependences with extremes, usually, minima) are not directly connected to the overloading of chromatographic columns, although they are observed in approaching to the overlo