Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Fast neurotransmitter release regulated by the endocytic scaffold intersectin. / Sakaba, Takeshi; Kononenko, Natalia L.; Bacetic, Jelena; Pechstein, Arndt; Schmoranzer, Jan; Yao, Lijun; Barth, Holger; Shupliakov, Oleg; Kobler, Oliver; Aktories, Klaus; Haucke, Volker.
в: Proceedings of the National Academy of Sciences of the United States of America, Том 110, № 20, 14.05.2013, стр. 8266-8271.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Fast neurotransmitter release regulated by the endocytic scaffold intersectin
AU - Sakaba, Takeshi
AU - Kononenko, Natalia L.
AU - Bacetic, Jelena
AU - Pechstein, Arndt
AU - Schmoranzer, Jan
AU - Yao, Lijun
AU - Barth, Holger
AU - Shupliakov, Oleg
AU - Kobler, Oliver
AU - Aktories, Klaus
AU - Haucke, Volker
PY - 2013/5/14
Y1 - 2013/5/14
N2 - Sustained fast neurotransmissionrequires therapid replenishment of release-ready synaptic vesicles (SVs) at presynaptic active zones. Although the machineries for exocytic fusion and for subsequent endocytic membrane retrieval have been well characterized, little is known about the mechanisms underlying the rapid recruitment of SVs to release sites. Here we show that the Down syndromeassociated endocytic scaffold protein intersectin 1 is a crucial factor for the recruitment of release-ready SVs. Genetic deletion of intersectin 1 expression or acute interference with intersectin function inhibited the replenishment of release-ready vesicles, resulting in short-term depression, without significantly affecting the rate of endocytic membrane retrieval. Acute perturbation experiments suggest that intersectin-mediated vesicle replenishment involves the association of intersectin with the fissioning enzyme dynamin andwith the actin regulatory GTPase CDC42. Our data indicate a role for the endocytic scaffold intersectin in fast neurotransmitter release, which may be of prime importance for information processing in the brain.
AB - Sustained fast neurotransmissionrequires therapid replenishment of release-ready synaptic vesicles (SVs) at presynaptic active zones. Although the machineries for exocytic fusion and for subsequent endocytic membrane retrieval have been well characterized, little is known about the mechanisms underlying the rapid recruitment of SVs to release sites. Here we show that the Down syndromeassociated endocytic scaffold protein intersectin 1 is a crucial factor for the recruitment of release-ready SVs. Genetic deletion of intersectin 1 expression or acute interference with intersectin function inhibited the replenishment of release-ready vesicles, resulting in short-term depression, without significantly affecting the rate of endocytic membrane retrieval. Acute perturbation experiments suggest that intersectin-mediated vesicle replenishment involves the association of intersectin with the fissioning enzyme dynamin andwith the actin regulatory GTPase CDC42. Our data indicate a role for the endocytic scaffold intersectin in fast neurotransmitter release, which may be of prime importance for information processing in the brain.
KW - Endocytosis
KW - Synaptic transmission
KW - Synaptic vesicle recruitment
UR - http://www.scopus.com/inward/record.url?scp=84877872053&partnerID=8YFLogxK
U2 - 10.1073/pnas.1219234110
DO - 10.1073/pnas.1219234110
M3 - Article
C2 - 23633571
AN - SCOPUS:84877872053
VL - 110
SP - 8266
EP - 8271
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 20
ER -
ID: 40828382