In this work we report two-step in situ laser-induced synthesis of bimetallic micro-sized struc-tures based on nickel and gold exhibiting good electrical conductivity and high electrocatalytic ac-tivity. This synthesis was carried out using consecutive deposition of nickel and gold on the sur-face of glass-ceramics upon laser irradiation at 532 nm from aqueous and organic solutions con-taining the salts of the corresponding metals. The electrochemical properties of the obtained nick-el-gold microsensor were investigated using voltammetric methods. We observed that the calcu-lated limit of detection (LOD) and sensitivity of the synthesized material with respect to glucose sensing are 1.8 □M and 2670 □A mM-1 cm-2, respectively.