TY - JOUR

T1 - Expression of the starfish complement component C3 gene homolog under the influence of bacterial lipopolysaccharide

AU - Mogilenko, D. A.

AU - Kudryavtsev, I. V.

AU - Orlov, S. V.

AU - Kharasova, A. D.

AU - Polevschikov, A. V.

N1 - Funding Information: This work was supported by the Russian Founda tion for Basic Research (project nos. 07 04 00084, 07 04 00095, and 04 08 00111).

PY - 2010/3

Y1 - 2010/3

N2 - A fragment of a homolog of the complement component C3 gene was cloned and sequenced from the starfish Asterias rubens. A phylogenetic analysis of the gene, termed ArC3-like, demonstrated its close similarity to the C3 gene homolog of deuterostome invertebrates. A high level of ArC3-like mRNA expression was observed in circulating cells (coelomocytes), a gut derivate (hepatopancreas), and the male gonad, but not in the stomach, female gonad, and rectal gland of A. rubens. ArC3-like gene expression was detected in all types of starfish coelomocytes, including lymphocyte-like cells and granular and nongranular amebocytes. Bacterial lipopolysaccharide (LPS) injected into the coelomic cavity of starfish increased the ArC3-like gene expression in coelomocytes and the hepatopancreas as compared to the control level (sterile sea water injection). The level of ArC3-like gene expression in coelomocytes in response to LPS reached its maximum 6 h after the injection and decreased to the baseline level 24 h after the injection. In the hepatopancreas, the level of ArC3-like gene expression reached its maximum 6-12 h after the LPS injection stimulation and remained high even 48 h after the injection. A long-term upregulation in response to LPS was demonstrated for the ArC3-like gene.

AB - A fragment of a homolog of the complement component C3 gene was cloned and sequenced from the starfish Asterias rubens. A phylogenetic analysis of the gene, termed ArC3-like, demonstrated its close similarity to the C3 gene homolog of deuterostome invertebrates. A high level of ArC3-like mRNA expression was observed in circulating cells (coelomocytes), a gut derivate (hepatopancreas), and the male gonad, but not in the stomach, female gonad, and rectal gland of A. rubens. ArC3-like gene expression was detected in all types of starfish coelomocytes, including lymphocyte-like cells and granular and nongranular amebocytes. Bacterial lipopolysaccharide (LPS) injected into the coelomic cavity of starfish increased the ArC3-like gene expression in coelomocytes and the hepatopancreas as compared to the control level (sterile sea water injection). The level of ArC3-like gene expression in coelomocytes in response to LPS reached its maximum 6 h after the injection and decreased to the baseline level 24 h after the injection. In the hepatopancreas, the level of ArC3-like gene expression reached its maximum 6-12 h after the LPS injection stimulation and remained high even 48 h after the injection. A long-term upregulation in response to LPS was demonstrated for the ArC3-like gene.

KW - Coelomocytes

KW - Complement component C3

KW - Echinoderms

KW - Innate immunity

KW - LPS

KW - Starfish

UR - http://www.scopus.com/inward/record.url?scp=77949290106&partnerID=8YFLogxK

U2 - 10.1134/S0026893310010097

DO - 10.1134/S0026893310010097

M3 - Article

AN - SCOPUS:77949290106

VL - 44

SP - 67

EP - 76

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 1

ER -