TY - JOUR

T1 - Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from Aplysina archeri (Aplysineidae: Verongiida) Demosponge

AU - Klinger, C.

AU - Żółtowska-Aksamitowska, S.

AU - Wysokowski , M.

AU - Tsurkan , M.V.

AU - Galli, R.

AU - Petrenko , J.

AU - Machałowski , T.

AU - Ereskovsky, Alexander

AU - Martinović , R.

AU - Muzychka , L.

AU - Muzychka , O.B.

AU - Bechmann , N.

AU - Ivanenko, Viatcheslav

AU - Schupp , P.J.

AU - Jesionowski , T.

AU - Giovine , M.

AU - Joseph , Y.

AU - Bornstein , S.R.

AU - Voronkina , A.

AU - Ehrlich, Hermann

PY - 2019/2/22

Y1 - 2019/2/22

N2 - Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of A. archeri and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene blue dye, crude oil, and blood, which is necessary for the application of such renewable 3D chitinous centimeter-sized scaffolds in diverse technological and biomedical fields.

AB - Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of A. archeri and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene blue dye, crude oil, and blood, which is necessary for the application of such renewable 3D chitinous centimeter-sized scaffolds in diverse technological and biomedical fields.

KW - Aplysina archeri

KW - Blood

KW - Bromotyrosines

KW - Chitin

KW - Crude oil

KW - Express method

KW - Marine sponges

KW - Methylene blue

KW - Scaffolds

KW - bromotyrosines

KW - PORIFERAN CHITIN

KW - methylene blue

KW - EXTRACTION

KW - scaffolds

KW - SURFACE

KW - MECHANICAL STRENGTH

KW - 1ST EVIDENCE

KW - HYDROGELS

KW - crude oil

KW - CHITOSAN

KW - PART II

KW - MARINE SPONGES

KW - blood

KW - chitin

KW - express method

KW - marine sponges

KW - SILICA

UR - http://www.scopus.com/inward/record.url?scp=85063227214&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/express-method-isolation-readytouse-3d-chitin-scaffolds-aplysina-archeri-aplysineidae-verongiida-dem

U2 - 10.3390/md17020131

DO - 10.3390/md17020131

M3 - Article

C2 - 30813373

VL - 17

JO - Marine Drugs

JF - Marine Drugs

SN - 1660-3397

IS - 2

M1 - 131

ER -