TY - CONF

T1 - Evaluation of non-specific CRISPR/Cas9 activity in a yeast model

AU - Шумега, Андрей Романович

AU - Степченкова, Елена Игоревна

AU - Инге-Вечтомов, Сергей Георгиевич

PY - 2023/12/4

Y1 - 2023/12/4

N2 - CRISPR/Cas9-based genome editing systems are widely used for genetic modification of different organisms. One of the drawbacks of CRISPR/Cas9 methods is the non-specific activity of Cas9, which can lead to accumulation of unwanted mutations in the edited genome [1]. Therefore, the development of in vivo models for high-throughput analysis of factors influencing the frequency of mutagenesis associated with the use of CRISPR/Cas9 technologies is a relevant task. Yeast Saccharomyces cerevisiae is a convenient object for developing such models [2]. Here we represent a yeast in vivo model that allows us to evaluate the effects of nucleotide sequence of the protospacer adjacent motif (PAM) and the guide RNA (gRNA) on the efficiency of binding between the gRNA/Cas9 complex and the target sequence in the genome. Since the Cas9 activity is lethal in cells lacking a donor sequence for homologous repair of double-strand breaks caused by this endonuclease, in the proposed test-system, the reduced efficiency of transformation by a plasmid encoding Cas9 and various gRNA variants reflects the efficiency of recognition of the target gene by the gRNA/Cas9 complex. To study the influence of different PAM variants, with a consensus of NGG, on CRISPR/ Cas9 activity, we obtained four isogenic strains that differ in their PAM sequence (AGG, TGG, CGG, GGG) in the codon 202 of the chromosomal copy of the reporter gene URA3. To evaluate the effect of incomplete matching between gRNA and the target site sequences, we propose using a series of plasmids based on the pML107 vector, encoding Cas9 and one of the 20 possible gRNA variants with single nucleotide substitutions at each of the 20 positions. The results obtained so far indicate the potential of the proposed approach.

AB - CRISPR/Cas9-based genome editing systems are widely used for genetic modification of different organisms. One of the drawbacks of CRISPR/Cas9 methods is the non-specific activity of Cas9, which can lead to accumulation of unwanted mutations in the edited genome [1]. Therefore, the development of in vivo models for high-throughput analysis of factors influencing the frequency of mutagenesis associated with the use of CRISPR/Cas9 technologies is a relevant task. Yeast Saccharomyces cerevisiae is a convenient object for developing such models [2]. Here we represent a yeast in vivo model that allows us to evaluate the effects of nucleotide sequence of the protospacer adjacent motif (PAM) and the guide RNA (gRNA) on the efficiency of binding between the gRNA/Cas9 complex and the target sequence in the genome. Since the Cas9 activity is lethal in cells lacking a donor sequence for homologous repair of double-strand breaks caused by this endonuclease, in the proposed test-system, the reduced efficiency of transformation by a plasmid encoding Cas9 and various gRNA variants reflects the efficiency of recognition of the target gene by the gRNA/Cas9 complex. To study the influence of different PAM variants, with a consensus of NGG, on CRISPR/ Cas9 activity, we obtained four isogenic strains that differ in their PAM sequence (AGG, TGG, CGG, GGG) in the codon 202 of the chromosomal copy of the reporter gene URA3. To evaluate the effect of incomplete matching between gRNA and the target site sequences, we propose using a series of plasmids based on the pML107 vector, encoding Cas9 and one of the 20 possible gRNA variants with single nucleotide substitutions at each of the 20 positions. The results obtained so far indicate the potential of the proposed approach.

KW - CRISPR/Cas9

KW - PAM

KW - Saccharomyces cerevisiae

UR - https://www.mendeley.com/catalogue/58daf6b0-20b2-3fb6-8007-2aa4cda04dc3/

UR - https://www.mendeley.com/catalogue/58daf6b0-20b2-3fb6-8007-2aa4cda04dc3/

U2 - 10.17816/ecogen567918

DO - 10.17816/ecogen567918

M3 - Abstract

Y2 - 3 October 2023 through 5 October 2023

ER -