TY - JOUR

T1 - Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

AU - Zhuk, A. S.

AU - Stepchenkova, E. I.

AU - Pavlov, Y. I.

AU - Inge-Vechtomov, S. G.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

AB - Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

KW - alpha-factor

KW - cdc28-4

KW - flow cytometry

KW - hydroxyurea

KW - nocodazole

KW - synchronize of cell division

KW - yeast Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=85018499854&partnerID=8YFLogxK

U2 - 10.1134/S1990519X17020110

DO - 10.1134/S1990519X17020110

M3 - Article

AN - SCOPUS:85018499854

VL - 11

SP - 111

EP - 122

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 2

ER -