TY - JOUR

T1 - Electrophoretic Determination of Carbohydrates in Samples of Natural Origin by an Indirect Detection Method

AU - Kolobova, E.A.

AU - Maliushevska, A.V.

AU - Kartsova, L.A.

N1 - Export Date: 11 March 2024 CODEN: JACTE Адрес для корреспонденции: Maliushevska, A.V.; St. Petersburg State University, Petrodvorets, Russian Federation; эл. почта: malushevskaa@gmail.com Сведения о финансировании: Russian Science Foundation, RSF, 19-13-00370 Текст о финансировании 1: The work was carried out with financial support from the Russian Science Foundation, project no. 19-13-00370. Пристатейные ссылки: Chen, Y.F., Li, M.Y., Wang, S.R., Peng, H.J., Reid, S., Ni, N.T., Fang, H., Xu, W.F., Carbohydrate biomarkers for future disease detection and treatment (2010) Sci. China Chem, 53, p. 3. , 1:CAS:528:DC%2BC3cXjslartr0%3D; Kiely, L.J., Hickey, R.M., (2022) Glycosylation, , New York, Humana; Nagy, G., Peng, T., Pohl, N.L.B., (2017) Anal. Methods, 9, p. 3579. , 1:CAS:528:DC%2BC2sXotlajtrs%3D, 28824713, 5558844; Kartsova, L.A., Maliushevska, A.V., Kolobova, E.A., (2023) J. Anal. Chem, 78, p. 156; Rovio, S., Yli-Kauhaluoma, J., Siren, H., (2007) Electrophoresis, 28, p. 3129. , 1:CAS:528:DC%2BD2sXhtFWmu7nP, 17661315; Alekseeva, A.V., Kartsova, L.A., Kazachishcheva, N.V., (2010) J. Anal. Chem, 65, p. 202. , 1:CAS:528:DC%2BC3cXisFOisro%3D; Schwaiger, H., Oefner, P.J., Huber, C., Grill, E., Bonn, G.K., (1994) Electrophoresis, 15, p. 941. , 1:CAS:528:DyaK2cXlsFamu78%3D, 7813400; Taga, A., Suzuki, S., Honda, S., (2001) J. Chromatogr. A, 911, p. 259. , 1:CAS:528:DC%2BD3MXht12htro%3D, 11293587; Soga, T., Ross, G.A., (1999) J. Chromatogr. A, 837, p. 231. , 1:CAS:528:DyaK1MXitlKjsbo%3D; Vaher, M., Koel, M., Kazarjan, J., Kaljurand, M., (2011) Electrophoresis, 32, p. 1068. , 1:CAS:528:DC%2BC3MXks1Wiu7k%3D, 21449068; Jiang, T.F., Chong, L., Yue, M.E., Wang, Y.H., Lv, Z.H., (2015) Food Anal. Methods, 8, p. 2588; Jager, A.V., Tonin, F.G., Tavares, M.F.M., (2007) J. Sep. Sci, 30, p. 586. , 1:CAS:528:DC%2BD2sXjslWqtL4%3D, 17444228; Dominguez, M.A., Jacksen, J., Emmer, A., Centurion, M.E., (2016) Microchem. J, 129, p. 1. , 1:CAS:528:DC%2BC28Xps1yktLw%3D; Warren, C.R., Adams, M.A., (2000) J. Exp. Bot, 51, p. 1147. , 1:CAS:528:DC%2BD3cXktVCksrc%3D, 10948242; Xu, X., Kok, W.T., Poppe, H., (1995) J. Chromatogr. A, 716, p. 231. , 1:CAS:528:DyaK2MXpsV2ltbo%3D; Lu, B., Westerlund, D., (1996) Electrophoresis, 17, p. 325. , 1:CAS:528:DyaK28XhslKjsb4%3D, 8900938; Gürel, A., Hızal, J., Öztekin, N., Erim, F.B., (2006) Chromatographia, 64, p. 321. , 1:CAS:528:DC%2BD2sXhslKiu78%3D; Mikkers, F.E.P., Everaerts, F.M., Verheggen, T.P.E.M., (1979) J. Chromatogr. A, 169, p. 1. , 1:CAS:528:DyaE1MXhsVamtr4%3D; Shamsi, S., (2006) Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation, , Hoboken, Wiley; Li, W., Zhang, M., Zhang, J., Han, Y., (2006) Front. Chem. China, 1, p. 438; Kolobova, E., Kartsova, L., Kravchenko, A., Bessonova, E., (2018) Talanta, 188, p. 183. , 1:CAS:528:DC%2BC1cXhtVGitrvM, 30029362; Blesic, M., Marques, M.H., Plechkova, N.V., Seddon, K.R., Rebelo, L.P.N., Lopes, A., (2007) Green Chem, 9, p. 481. , 1:CAS:528:DC%2BD2sXmslyqt7w%3D; Kaper, H., Smarsly, B., (2006) Z. Phys. Chem, 220, p. 1455. , 1:CAS:528:DC%2BD2sXmsFCqsQ%3D%3D

PY - 2024/2/1

Y1 - 2024/2/1

N2 - Abstract: Versions of the electrophoretic determination of neutral carbohydrates by a method of indirect detection using acridone acetic and folic acids as absorbing additives (AA) are proposed. The effects of the nature and concentration of AA, alkali, and various modifiers (cetyltrimethylammonium bromide, CTAB) and ionic liquids (1-dodecyl-3-methylimidazolium chloride and 1-hexadecyl-3-methylimidazolium chloride) on the electrophoretic parameters of the migration of analytes are studied. The lowest limits of detection for carbohydrates are achieved in a background electrolyte containing 2.5 mM acridone acetic acid, 75 mM KOH, 0.5 mM CTAB, and 5 vol % MeOH, and amount to 4−10 μg/mL with an efficiency of up to 350 thousand t.p. Under the conditions found, samples of buckwheat honey and human blood plasma are analyzed. It is found that in analyzing food products, it is preferable to use folic acid as a AA, because it provides the best selectivity for the separation of carbohydrates: for the sucralose−sucrose pair, the resolution factor is 7.6. © Pleiades Publishing, Ltd. 2024. ISSN 1061-9348, Journal of Analytical Chemistry, 2024, Vol. 79, No. 2, pp. 224–232. Pleiades Publishing, Ltd., 2024.

AB - Abstract: Versions of the electrophoretic determination of neutral carbohydrates by a method of indirect detection using acridone acetic and folic acids as absorbing additives (AA) are proposed. The effects of the nature and concentration of AA, alkali, and various modifiers (cetyltrimethylammonium bromide, CTAB) and ionic liquids (1-dodecyl-3-methylimidazolium chloride and 1-hexadecyl-3-methylimidazolium chloride) on the electrophoretic parameters of the migration of analytes are studied. The lowest limits of detection for carbohydrates are achieved in a background electrolyte containing 2.5 mM acridone acetic acid, 75 mM KOH, 0.5 mM CTAB, and 5 vol % MeOH, and amount to 4−10 μg/mL with an efficiency of up to 350 thousand t.p. Under the conditions found, samples of buckwheat honey and human blood plasma are analyzed. It is found that in analyzing food products, it is preferable to use folic acid as a AA, because it provides the best selectivity for the separation of carbohydrates: for the sucralose−sucrose pair, the resolution factor is 7.6. © Pleiades Publishing, Ltd. 2024. ISSN 1061-9348, Journal of Analytical Chemistry, 2024, Vol. 79, No. 2, pp. 224–232. Pleiades Publishing, Ltd., 2024.

KW - absorbing additives

KW - capillary electrophoresis

KW - carbohydrates

KW - indirect detection

KW - Additives

KW - Ascorbic acid

KW - Blood

KW - Carbohydrates

KW - Chlorine compounds

KW - Electrolytes

KW - Food products

KW - Ionic liquids

KW - Potassium hydroxide

KW - Absorbing additive

KW - Acridones

KW - Additive alkali

KW - Analytes

KW - Cetyltrimethylammonium bromide

KW - Detection methods

KW - Folic acids

KW - Indirect detection

KW - Lower limits of detections

KW - Methylimidazolium chloride

KW - Capillary electrophoresis

UR - https://www.mendeley.com/catalogue/b2e2a87e-fdd0-374e-800f-2635975e87d6/

U2 - 10.1134/s1061934824020102

DO - 10.1134/s1061934824020102

M3 - статья

VL - 79

SP - 224

EP - 232

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 2

ER -