Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems

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Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems. / Dvinskikh, Sergey V.; Castro, Vasco; Sandström, Dick.

в: Physical Chemistry Chemical Physics, Том 7, № 4, 21.02.2005, стр. 607-613.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{2d847e955b414f7883420435689068b4,

title = "Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems",

abstract = "Recently (Dvinskikh et al., J. Magn. Reson., 2003, 164, 165 and Dvinskikh et al., J. Magn. Reson., 2004, 168, 194), some of us introduced two efficient solid-state NMR techniques for the determination of heteronuclear dipolar couplings under magic-angle spinning (MAS). These two-dimensional (2D) recoupling methods have been applied previously to simple amino acids, and to columnar systems with high positional and orientational order. In this work, we show that the 2D MAS sequences produce unparalleled 1H-13C dipolar resolution in unoriented lipid membranes. The recoupling experiments were applied to hydrated dimyristoylphosphatidylcholine (DMPC) in the liquid-crystalline Lα phase, and the results agreed well with previous NMR investigations using specifically deuterated phospholipids.",

author = "Dvinskikh, {Sergey V.} and Vasco Castro and Dick Sandstr{\"o}m",

year = "2005",

month = feb,

day = "21",

doi = "10.1039/b418131j",

language = "English",

volume = "7",

pages = "607--613",

journal = "Physical Chemistry Chemical Physics",

issn = "1463-9076",

publisher = "Royal Society of Chemistry",

number = "4",

}

RIS

TY - JOUR

T1 - Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems

AU - Dvinskikh, Sergey V.

AU - Castro, Vasco

AU - Sandström, Dick

PY - 2005/2/21

Y1 - 2005/2/21

N2 - Recently (Dvinskikh et al., J. Magn. Reson., 2003, 164, 165 and Dvinskikh et al., J. Magn. Reson., 2004, 168, 194), some of us introduced two efficient solid-state NMR techniques for the determination of heteronuclear dipolar couplings under magic-angle spinning (MAS). These two-dimensional (2D) recoupling methods have been applied previously to simple amino acids, and to columnar systems with high positional and orientational order. In this work, we show that the 2D MAS sequences produce unparalleled 1H-13C dipolar resolution in unoriented lipid membranes. The recoupling experiments were applied to hydrated dimyristoylphosphatidylcholine (DMPC) in the liquid-crystalline Lα phase, and the results agreed well with previous NMR investigations using specifically deuterated phospholipids.

AB - Recently (Dvinskikh et al., J. Magn. Reson., 2003, 164, 165 and Dvinskikh et al., J. Magn. Reson., 2004, 168, 194), some of us introduced two efficient solid-state NMR techniques for the determination of heteronuclear dipolar couplings under magic-angle spinning (MAS). These two-dimensional (2D) recoupling methods have been applied previously to simple amino acids, and to columnar systems with high positional and orientational order. In this work, we show that the 2D MAS sequences produce unparalleled 1H-13C dipolar resolution in unoriented lipid membranes. The recoupling experiments were applied to hydrated dimyristoylphosphatidylcholine (DMPC) in the liquid-crystalline Lα phase, and the results agreed well with previous NMR investigations using specifically deuterated phospholipids.

UR - http://www.scopus.com/inward/record.url?scp=14644399800&partnerID=8YFLogxK

U2 - 10.1039/b418131j

DO - 10.1039/b418131j

M3 - Article

C2 - 19787876

AN - SCOPUS:14644399800

VL - 7

SP - 607

EP - 613

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

SN - 1463-9076

IS - 4

ER -

ID: 48948483