TY - JOUR

T1 - Effects of lidocaine on adult zebrafish behavior and brain acetylcholinesterase following peripheral and systemic administration

AU - de Abreu, Murilo S.

AU - Giacomini, Ana C.V.V.

AU - dos Santos, Bruna E.

AU - Genario, Rafael

AU - Marchiori, Natalia I.

AU - Rosa, Larissa G.da

AU - Kalueff, Allan V.

PY - 2019/1/23

Y1 - 2019/1/23

N2 - Lidocaine is a voltage-gated Na+ channel blocker, commonly used as a fast-acting local and general anesthetic. Lidocaine also has central action, affecting behavior both clinically and in animal models. Adult zebrafish are rapidly becoming a critical novel model organism in translational neuroscience research. Here, we examine the effects of acute peripheral (lateral line application, 4%) and systemic (water immersion, 1, 5 and 10 mg/L) administration of lidocaine on adult zebrafish behavior tested in the novel tank test. Overall, the drug evoked hypolocomotor effect when applied systemically (at 10 mg/L) and peripherally. Peripheral lidocaine also reduced top exploration in the novel tank test (vs. sham), suggesting anxiogenic-like effect of the lateral line blockage, Our findings show the importance of the lateral line system in driving adult zebrafish locomotion, and suggest sedative-like effects of systemic lidocaine in aduld zebrafish. In addition, reflecting the role of central cholinergic contribution in lidocaine action, brain acetylcholinesterase (AChE) activity was lower following peripheral and systemic administration of lidocaine at behaviorally active doses. Collectively, our data support the effects of lidocaine on behavioral responses in zebrafish, and reinforce the growing utility of this aquatic model to screen various CNS drugs.

AB - Lidocaine is a voltage-gated Na+ channel blocker, commonly used as a fast-acting local and general anesthetic. Lidocaine also has central action, affecting behavior both clinically and in animal models. Adult zebrafish are rapidly becoming a critical novel model organism in translational neuroscience research. Here, we examine the effects of acute peripheral (lateral line application, 4%) and systemic (water immersion, 1, 5 and 10 mg/L) administration of lidocaine on adult zebrafish behavior tested in the novel tank test. Overall, the drug evoked hypolocomotor effect when applied systemically (at 10 mg/L) and peripherally. Peripheral lidocaine also reduced top exploration in the novel tank test (vs. sham), suggesting anxiogenic-like effect of the lateral line blockage, Our findings show the importance of the lateral line system in driving adult zebrafish locomotion, and suggest sedative-like effects of systemic lidocaine in aduld zebrafish. In addition, reflecting the role of central cholinergic contribution in lidocaine action, brain acetylcholinesterase (AChE) activity was lower following peripheral and systemic administration of lidocaine at behaviorally active doses. Collectively, our data support the effects of lidocaine on behavioral responses in zebrafish, and reinforce the growing utility of this aquatic model to screen various CNS drugs.

KW - Anxiety

KW - Emotional behavior

KW - Lidocaine

KW - Sedation

KW - Zebrafish

KW - Exploratory Behavior/drug effects

KW - Lidocaine/administration & dosage

KW - Acetylcholinesterase/metabolism

KW - Male

KW - Anxiety/chemically induced

KW - Brain/drug effects

KW - Animals

KW - Behavior, Animal/drug effects

KW - Female

KW - Models, Animal

KW - Swimming

UR - http://www.scopus.com/inward/record.url?scp=85056711928&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2018.11.004

DO - 10.1016/j.neulet.2018.11.004

M3 - Article

C2 - 30399397

AN - SCOPUS:85056711928

VL - 692

SP - 181

EP - 186

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

ER -