TY - JOUR

T1 - Effects of Deletions in Pichia pastoris RTG Genes on Phenotype and AOX1 Expression

AU - Rumyantsev, A.M.

AU - Soloviev, G. A.

AU - Slepchenkov, A. V.

AU - Sambuk, E. V.

PY - 2018/5

Y1 - 2018/5

N2 - Methylotrophic yeast Pichia pastoris is an object of modern biotechnology. Decisive understanding of gene regulation mechanisms is essential for successful protein production. In this study, we investigated the effect of deletions in P. pastoris genes encoding proteins, homologous to S. serevisiae Rtg1p, Rtg 2p, Msn2p and Msn4p. It was shown, that deletion in PpRTG1 gene results in inability of P. pastoris to grow on medium with methanol as a carbon source and ammonium sulfate as a source of nitrogen. We also demonstrate that deletions in PpRTG1 and PpRTG2 decrease activity of AOX1 promoter.

AB - Methylotrophic yeast Pichia pastoris is an object of modern biotechnology. Decisive understanding of gene regulation mechanisms is essential for successful protein production. In this study, we investigated the effect of deletions in P. pastoris genes encoding proteins, homologous to S. serevisiae Rtg1p, Rtg 2p, Msn2p and Msn4p. It was shown, that deletion in PpRTG1 gene results in inability of P. pastoris to grow on medium with methanol as a carbon source and ammonium sulfate as a source of nitrogen. We also demonstrate that deletions in PpRTG1 and PpRTG2 decrease activity of AOX1 promoter.

KW - ACID-PHOSPHATASES

KW - AOX1

KW - COMPARATIVE GENOMICS

KW - EXPRESSION

KW - GROWTH-CONTROL

KW - METHANOL UTILIZATION PATHWAY

KW - MITOCHONDRIA

KW - PROTEIN-PRODUCTION

KW - Pichia pastoris

KW - Retrograde Regulation Pathway

KW - SACCHAROMYCES-CEREVISIAE

KW - TOR

KW - Tor-Kinase

KW - YEAST

U2 - 10.4236/aim.2018.85029

DO - 10.4236/aim.2018.85029

M3 - Article

VL - 8

SP - 439

EP - 450

JO - Advances in Microbiology

JF - Advances in Microbiology

SN - 2165-3402

IS - 5

ER -