TY - JOUR

T1 - Effects of 1,2-dimethylhydrazine on barrier properties of rat large intestine and ipec-j2 cells

AU - Bekusova, Viktoria

AU - Droessler, Linda

AU - Amasheh, Salah

AU - Markov, Alexander G.

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/9/24

Y1 - 2021/9/24

N2 - Colon cancer is accompanied by a decrease of epithelial barrier properties, which are determined by tight junction (TJ) proteins between adjacent epithelial cells. The aim of the current study was to analyze the expression of TJ proteins in a rat model of 1,2-dimethylhydrazine (DMH)-induced colorectal cancer, as well as the barrier properties and TJ protein expression of IPEC-J2 cell monolayers after incubation with DMH. Transepithelial electrical resistance and paracellular permeability for sodium fluorescein of IPEC-J2 were examined by an epithelial volt/ohm meter and spectrophotometry. The expression and localization of TJ proteins were analyzed by immunoblotting and immunohistochemistry. In the colonic tumors of rats with DMH-induced carcinogenesis, the expression of claudin-3 and-4 was significantly increased compared to controls. The transepithelial electrical resistance of IPEC-J2 cells increased, while paracellular permeability for sodium fluorescein decreased, accompanied by an increased expression of claudin-4. The increase of claudin-4 in rat colon after chronic DMH exposure was consistent with the acute effect of DMH on IPEC-J2 cells, which may indicate an essential role of this protein in colorectal cancer development.

AB - Colon cancer is accompanied by a decrease of epithelial barrier properties, which are determined by tight junction (TJ) proteins between adjacent epithelial cells. The aim of the current study was to analyze the expression of TJ proteins in a rat model of 1,2-dimethylhydrazine (DMH)-induced colorectal cancer, as well as the barrier properties and TJ protein expression of IPEC-J2 cell monolayers after incubation with DMH. Transepithelial electrical resistance and paracellular permeability for sodium fluorescein of IPEC-J2 were examined by an epithelial volt/ohm meter and spectrophotometry. The expression and localization of TJ proteins were analyzed by immunoblotting and immunohistochemistry. In the colonic tumors of rats with DMH-induced carcinogenesis, the expression of claudin-3 and-4 was significantly increased compared to controls. The transepithelial electrical resistance of IPEC-J2 cells increased, while paracellular permeability for sodium fluorescein decreased, accompanied by an increased expression of claudin-4. The increase of claudin-4 in rat colon after chronic DMH exposure was consistent with the acute effect of DMH on IPEC-J2 cells, which may indicate an essential role of this protein in colorectal cancer development.

KW - 1,2-dimethylhydrazine

KW - Barrier properties

KW - Colorectal cancer

KW - IPEC-J2

KW - Tight junction proteins

KW - Transepithelial electrical resistance

KW - SODIUM

KW - colorectal cancer

KW - PERMEABILITY

KW - MOUSE

KW - COLON CARCINOGENESIS

KW - PROLIFERATION

KW - TIGHT JUNCTION

KW - TRANSPORT

KW - barrier properties

KW - EPITHELIAL-CELLS

KW - transepithelial electrical resistance

KW - <p>1,2-dimethylhydrazine & nbsp;</p>

KW - tight junction proteins

KW - CLAUDIN-4 EXPRESSION

KW - UP-REGULATION

UR - http://www.scopus.com/inward/record.url?scp=85115662270&partnerID=8YFLogxK

U2 - 10.3390/ijms221910278

DO - 10.3390/ijms221910278

M3 - Article

C2 - 34638619

AN - SCOPUS:85115662270

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 19

M1 - 10278

ER -