Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae

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Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae. / Popova, Yu G.; Padkina, M. V.; Sambuk, E. V.

в: Russian Journal of Genetics, Том 36, № 12, 01.01.2000, стр. 1364-1369.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{936c7dd54e3d42f88f6ec7f803386fb0,

title = "Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae",

abstract = "Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.",

author = "Popova, {Yu G.} and Padkina, {M. V.} and Sambuk, {E. V.}",

year = "2000",

month = jan,

day = "1",

doi = "10.1023/A:1009059005287",

language = "English",

volume = "36",

pages = "1364--1369",

journal = "Russian Journal of Genetics",

issn = "1022-7954",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "12",

}

RIS

TY - JOUR

T1 - Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae

AU - Popova, Yu G.

AU - Padkina, M. V.

AU - Sambuk, E. V.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.

AB - Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.

UR - http://www.scopus.com/inward/record.url?scp=22044451080&partnerID=8YFLogxK

U2 - 10.1023/A:1009059005287

DO - 10.1023/A:1009059005287

M3 - Article

AN - SCOPUS:22044451080

VL - 36

SP - 1364

EP - 1369

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 12

ER -

ID: 36922140