Результаты исследований: Научные публикации в периодических изданиях › статья
Dynamics of satellite binding protein CENP-B and telomere binding protein TRF2/MTBP in the nuclei of mouse spermatogenic line. / Dolnik, AV; Pochukalina, GN; Parfenov, VN; Karpushev, AV; Podgornaya, OI; Voronin, AP.
в: Cell Biology International, Том 31, № 4, 2007, стр. 316-329.Результаты исследований: Научные публикации в периодических изданиях › статья
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TY - JOUR
T1 - Dynamics of satellite binding protein CENP-B and telomere binding protein TRF2/MTBP in the nuclei of mouse spermatogenic line
AU - Dolnik, AV
AU - Pochukalina, GN
AU - Parfenov, VN
AU - Karpushev, AV
AU - Podgornaya, OI
AU - Voronin, AP.
PY - 2007
Y1 - 2007
N2 - The location of centromeric protein CENP-B and telomeric protein TRF2/MTBP in the mouse spermatogenic line has been studied using indirect immunofluorescent and immunoelectron microscopy. CENP-B localized to the heterochromatic parts of the nuclei at meiotic stages. A clearly distinct chromocenter forms in the nucleus at stages 3-4 of spermatid maturation; CENP-B localizes in it and in the area adjacent to the future acrosome. CENP-B localization in the subacrosomal area and in the chromocenters' periphery demonstrates that centromeres are organized in two groups in mouse spermatozoa, unlike human centromeres. TRF2/MTBP concentrates around the forming chromocenter at spermiogenesis early stages. The TRF2/MTBP main signal migrates into the area of acrosomal membrane at the course of spermatozoon maturation. TRF2/MTBP never localizes inside the synaptonemal complex but can be found in the areas where the synaptonemal complex attaches to the nuclear envelope. At the pachytene and diplotene stages when chromosome
AB - The location of centromeric protein CENP-B and telomeric protein TRF2/MTBP in the mouse spermatogenic line has been studied using indirect immunofluorescent and immunoelectron microscopy. CENP-B localized to the heterochromatic parts of the nuclei at meiotic stages. A clearly distinct chromocenter forms in the nucleus at stages 3-4 of spermatid maturation; CENP-B localizes in it and in the area adjacent to the future acrosome. CENP-B localization in the subacrosomal area and in the chromocenters' periphery demonstrates that centromeres are organized in two groups in mouse spermatozoa, unlike human centromeres. TRF2/MTBP concentrates around the forming chromocenter at spermiogenesis early stages. The TRF2/MTBP main signal migrates into the area of acrosomal membrane at the course of spermatozoon maturation. TRF2/MTBP never localizes inside the synaptonemal complex but can be found in the areas where the synaptonemal complex attaches to the nuclear envelope. At the pachytene and diplotene stages when chromosome
KW - Spermatogenesis
KW - Acrosome
KW - Cell nucleus
KW - Chromatin
KW - TRF2
KW - CENP-B
U2 - 10.1016/j.cellbi.2007.01.017
DO - 10.1016/j.cellbi.2007.01.017
M3 - статья
VL - 31
SP - 316
EP - 329
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 4
ER -
ID: 5444936