TY - JOUR

T1 - Dual embryonic origin of the hyobranchial apparatus in the Mexican Axolotl (Ambystoma mexicanum)

AU - Davidian, A.

AU - Malashichev, Y.

PY - 2013

Y1 - 2013

N2 - Background. Traditionally, the cartilaginous viscerocranium of vertebrates is considered as neural crest (NC)-derived. Morphological work carried out on amphibian embryos in the first half of XX century suggested potentially mesodermal origin for some hyobranchial elements. Since then, the embryonic sources of the hyobranchial apparatus in amphibians had not been investigated due to the lack of an appropriate long-term labelling system. Methods. We performed homotopic transplantations of neural folds along with majority of cells of the presumptive NC, and/or fragments of the head lateral plate mesoderm (LPM) from transgenic GFP+ into white embryos. Results. In these experiments the NC-derived GFP+ cells contributed to all hyobranchial elements, except for basibranchial 2, whereas the grafting of GFP+ head mesoderm led to a reverse labeling result. The grafting of only the most ventral part of the head LPM resulted in marking of the basibranchial 2 and the heart myocardium, implying their origin from a comm

AB - Background. Traditionally, the cartilaginous viscerocranium of vertebrates is considered as neural crest (NC)-derived. Morphological work carried out on amphibian embryos in the first half of XX century suggested potentially mesodermal origin for some hyobranchial elements. Since then, the embryonic sources of the hyobranchial apparatus in amphibians had not been investigated due to the lack of an appropriate long-term labelling system. Methods. We performed homotopic transplantations of neural folds along with majority of cells of the presumptive NC, and/or fragments of the head lateral plate mesoderm (LPM) from transgenic GFP+ into white embryos. Results. In these experiments the NC-derived GFP+ cells contributed to all hyobranchial elements, except for basibranchial 2, whereas the grafting of GFP+ head mesoderm led to a reverse labeling result. The grafting of only the most ventral part of the head LPM resulted in marking of the basibranchial 2 and the heart myocardium, implying their origin from a comm

KW - Ambystoma mexicanum

KW - Mexican axolotl

KW - neural crest

KW - head lateral plate mesoderm

KW - branchial arches

KW - viscerocranium

KW - hyobranchial apparatus

KW - basibranchial 2

U2 - 10.1387/ijdb.130213ym

DO - 10.1387/ijdb.130213ym

M3 - Article

VL - 57

SP - 821

EP - 828

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

SN - 0214-6282

IS - 11/12

ER -