TY - JOUR

T1 - Drosophila lysophospholipase gene swiss cheese is required for survival and reproduction

AU - Melentev, Pavel A.

AU - Sharapenkov, Eduard G.

AU - Surina, Nina V.

AU - Ivanova, Ekaterina A.

AU - Ryabova, Elena V.

AU - Sarantseva, Svetlana V.

N1 - Melentev, P.A.; Sharapenkov, E.G.; Surina, N.V.; Ivanova, E.A.; Ryabova, E.V.; Sarantseva, S.V. Drosophila Lysophospholipase Gene swiss cheese Is Required for Survival and Reproduction. Insects 2022, 13, 14. https://doi.org/10.3390/insects13010014

PY - 2022/1

Y1 - 2022/1

N2 - Drosophila melanogaster is one of the most famous insects in biological research. It is widely used to analyse functions of different genes. The phosphatidylcholine lysophospholipase gene swiss cheese was initially shown to be important in the fruit fly nervous system. However, the role of this gene in non-nervous cell types has not been elucidated yet, and the evolutional explanation for the conservation of its function remains elusive. In this study, we analyse expression pattern and some aspects of the role of the swiss cheese gene in the fitness of Drosophila melanogaster. We describe the spatiotemporal expression of swiss cheese throughout the fly development and analyse the survival and productivity of swiss cheese mutants. We found swiss cheese to be expressed in salivary glands, midgut, Malpighian tubes, adipocytes, and male reproductive system. Dysfunction of swiss cheese results in severe pupae and imago lethality and decline of fertility, which is impressive in males. The latter is accompanied with abnormalities of male locomotor activity and courtship behaviour, accumulation of lipid droplets in testis cyst cells and decrease in spermatozoa motility. These results suggest that normal swiss cheese is important for Drosophila melanogaster fitness due to its necessity for both specimen survival and their reproductive success.

AB - Drosophila melanogaster is one of the most famous insects in biological research. It is widely used to analyse functions of different genes. The phosphatidylcholine lysophospholipase gene swiss cheese was initially shown to be important in the fruit fly nervous system. However, the role of this gene in non-nervous cell types has not been elucidated yet, and the evolutional explanation for the conservation of its function remains elusive. In this study, we analyse expression pattern and some aspects of the role of the swiss cheese gene in the fitness of Drosophila melanogaster. We describe the spatiotemporal expression of swiss cheese throughout the fly development and analyse the survival and productivity of swiss cheese mutants. We found swiss cheese to be expressed in salivary glands, midgut, Malpighian tubes, adipocytes, and male reproductive system. Dysfunction of swiss cheese results in severe pupae and imago lethality and decline of fertility, which is impressive in males. The latter is accompanied with abnormalities of male locomotor activity and courtship behaviour, accumulation of lipid droplets in testis cyst cells and decrease in spermatozoa motility. These results suggest that normal swiss cheese is important for Drosophila melanogaster fitness due to its necessity for both specimen survival and their reproductive success.

KW - Cyst cells

KW - Drosophila melanogaster

KW - Fertility

KW - Lipid droplets

KW - Lysophospholipase

KW - NTE

KW - PNPLA6

KW - Spermatozoa

KW - Swiss cheese

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=85121842116&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/07b584cb-2b41-35ff-a30a-deb3c9c68548/

U2 - 10.3390/insects13010014

DO - 10.3390/insects13010014

M3 - Article

AN - SCOPUS:85121842116

VL - 13

JO - Insects

JF - Insects

SN - 2075-4450

IS - 1

M1 - 14

ER -