DNA complexes with polycations useful for delivery of DNA into cells › Научные исследования в СПбГУ

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DNA complexes with polycations useful for delivery of DNA into cells. / Kas'yanenko, N. A.; Zakharova, N. B.; Mukhin, D. A.; Slita, A. V.; Nazarova, O. V.; Leont'eva, E. A.; Panarin, E. F.

в: Biophysics, Том 53, № 1, 02.2008, стр. 31-37.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Kas'yanenko, NA, Zakharova, NB, Mukhin, DA, Slita, AV, Nazarova, OV, Leont'eva, EA & Panarin, EF 2008, 'DNA complexes with polycations useful for delivery of DNA into cells', Biophysics, Том. 53, № 1, стр. 31-37. https://doi.org/10.1134/S0006350908010065

APA

Kas'yanenko, N. A., Zakharova, N. B., Mukhin, D. A., Slita, A. V., Nazarova, O. V., Leont'eva, E. A., & Panarin, E. F. (2008). DNA complexes with polycations useful for delivery of DNA into cells. Biophysics, 53(1), 31-37. https://doi.org/10.1134/S0006350908010065

Vancouver

Kas'yanenko NA, Zakharova NB, Mukhin DA, Slita AV, Nazarova OV, Leont'eva EA и пр. DNA complexes with polycations useful for delivery of DNA into cells. Biophysics. 2008 Февр.;53(1):31-37. https://doi.org/10.1134/S0006350908010065

Author

Kas'yanenko, N. A. ; Zakharova, N. B. ; Mukhin, D. A. ; Slita, A. V. ; Nazarova, O. V. ; Leont'eva, E. A. ; Panarin, E. F. / DNA complexes with polycations useful for delivery of DNA into cells. в: Biophysics. 2008 ; Том 53, № 1. стр. 31-37.

BibTeX

@article{225c2091d2b84e25855a32206cab7dae,

title = "DNA complexes with polycations useful for delivery of DNA into cells",

abstract = "Generation and physicochemical properties of complexes formed by high-molecular thymus DNA and plasmid DNA with synthetic polymers of (dimethyl amino)ethyl methacrylate, (diethyl amino)ethyl methacrylate, and poly(vinyl amine) were studied in solutions of different ionic strength using low-gradient viscometry, electrophoresis, circular dichroism, spectrophotometry, and dynamic light scattering. The complexes were tested for toxicity with T98G cell cultures. Condensation of DNA was shown to occur when the ratio of charged groups in the polycations and DNA exceeded unity. This condensation manifested itself as an increase in the optical density of DNA solutions. Condensation-associated changes in the dimensions of DNA molecules were determined, and phase diagrams of DNA-polycation systems were analyzed in the presence of NaCl. MTT analysis revealed no toxicity of these complexes.",

keywords = "DNA, DNA complexes with synthetic polymers, DNA condensation, Phase diagrams",

author = "Kas'yanenko, {N. A.} and Zakharova, {N. B.} and Mukhin, {D. A.} and Slita, {A. V.} and Nazarova, {O. V.} and Leont'eva, {E. A.} and Panarin, {E. F.}",

year = "2008",

month = feb,

doi = "10.1134/S0006350908010065",

language = "English",

volume = "53",

pages = "31--37",

journal = "Biophysics (Russian Federation)",

issn = "0006-3509",

publisher = "Springer Nature",

number = "1",

}

RIS

TY - JOUR

T1 - DNA complexes with polycations useful for delivery of DNA into cells

AU - Kas'yanenko, N. A.

AU - Zakharova, N. B.

AU - Mukhin, D. A.

AU - Slita, A. V.

AU - Nazarova, O. V.

AU - Leont'eva, E. A.

AU - Panarin, E. F.

PY - 2008/2

Y1 - 2008/2

N2 - Generation and physicochemical properties of complexes formed by high-molecular thymus DNA and plasmid DNA with synthetic polymers of (dimethyl amino)ethyl methacrylate, (diethyl amino)ethyl methacrylate, and poly(vinyl amine) were studied in solutions of different ionic strength using low-gradient viscometry, electrophoresis, circular dichroism, spectrophotometry, and dynamic light scattering. The complexes were tested for toxicity with T98G cell cultures. Condensation of DNA was shown to occur when the ratio of charged groups in the polycations and DNA exceeded unity. This condensation manifested itself as an increase in the optical density of DNA solutions. Condensation-associated changes in the dimensions of DNA molecules were determined, and phase diagrams of DNA-polycation systems were analyzed in the presence of NaCl. MTT analysis revealed no toxicity of these complexes.

AB - Generation and physicochemical properties of complexes formed by high-molecular thymus DNA and plasmid DNA with synthetic polymers of (dimethyl amino)ethyl methacrylate, (diethyl amino)ethyl methacrylate, and poly(vinyl amine) were studied in solutions of different ionic strength using low-gradient viscometry, electrophoresis, circular dichroism, spectrophotometry, and dynamic light scattering. The complexes were tested for toxicity with T98G cell cultures. Condensation of DNA was shown to occur when the ratio of charged groups in the polycations and DNA exceeded unity. This condensation manifested itself as an increase in the optical density of DNA solutions. Condensation-associated changes in the dimensions of DNA molecules were determined, and phase diagrams of DNA-polycation systems were analyzed in the presence of NaCl. MTT analysis revealed no toxicity of these complexes.

KW - DNA

KW - DNA complexes with synthetic polymers

KW - DNA condensation

KW - Phase diagrams

UR - http://www.scopus.com/inward/record.url?scp=47649097432&partnerID=8YFLogxK

U2 - 10.1134/S0006350908010065

DO - 10.1134/S0006350908010065

M3 - Article

AN - SCOPUS:47649097432

VL - 53

SP - 31

EP - 37

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

SN - 0006-3509

IS - 1

ER -

ID: 73390553