Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
DNA complexed with oppositely charged amphiphile in low-polar organic solvents. / Sergeyev, V. G.; Pyshkina, O. A.; Lezov, A. V.; Mel'nikov, A. B.; Ryumtsev, E. I.; Zezin, A. B.; Kabanov, V. A.
в: Langmuir, Том 15, № 13, 1999, стр. 4434-4440.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - DNA complexed with oppositely charged amphiphile in low-polar organic solvents
AU - Sergeyev, V. G.
AU - Pyshkina, O. A.
AU - Lezov, A. V.
AU - Mel'nikov, A. B.
AU - Ryumtsev, E. I.
AU - Zezin, A. B.
AU - Kabanov, V. A.
PY - 1999
Y1 - 1999
N2 - The stoichiometric complexes of the DNA samples of rather different molecular masses: 300-500 b.p. (DNA1) and 5000-10000 b.p. (DNA2) with dodecyldimethylammonium (DODA) chloride as a cationic amphiphile were prepared. DNA1-DODA and DNA2-DODA complexes are soluble in low polar organic solvents, in particular, in chloroform, as well as the complexes of ordinary linear polyelectrolytes with micelle-forming ionic surfactants. The solution behavior of the DNA-DODA complexes was studied by ultracentrifugation, viscometry, isothermal diffusion, and electrical birefringence techniques. It has been found that the DNA-DODA complex species in their dilute solutions in chloroform and a chloroform-acetone mixture (2:1) are represented by individual complex macromolecules retaining a double spirality, but strongly compacted. The latter is contrasting with other polyelectrolyte-surfactant complexes, which behave in chloroform as in a good solvent, taking expanded random coil conformations. The possible reason of such a basic difference is discussed and considered to be the result of an inherent tendency of the DNA double helix to be compacted if not ionized. This tendency has been detected due to a unique compromise between the thermodynamic solubility and complete electric neutrality of DNA-surfactant complex macromolecules in low polar organic solvent.
AB - The stoichiometric complexes of the DNA samples of rather different molecular masses: 300-500 b.p. (DNA1) and 5000-10000 b.p. (DNA2) with dodecyldimethylammonium (DODA) chloride as a cationic amphiphile were prepared. DNA1-DODA and DNA2-DODA complexes are soluble in low polar organic solvents, in particular, in chloroform, as well as the complexes of ordinary linear polyelectrolytes with micelle-forming ionic surfactants. The solution behavior of the DNA-DODA complexes was studied by ultracentrifugation, viscometry, isothermal diffusion, and electrical birefringence techniques. It has been found that the DNA-DODA complex species in their dilute solutions in chloroform and a chloroform-acetone mixture (2:1) are represented by individual complex macromolecules retaining a double spirality, but strongly compacted. The latter is contrasting with other polyelectrolyte-surfactant complexes, which behave in chloroform as in a good solvent, taking expanded random coil conformations. The possible reason of such a basic difference is discussed and considered to be the result of an inherent tendency of the DNA double helix to be compacted if not ionized. This tendency has been detected due to a unique compromise between the thermodynamic solubility and complete electric neutrality of DNA-surfactant complex macromolecules in low polar organic solvent.
UR - http://www.scopus.com/inward/record.url?scp=0032658415&partnerID=8YFLogxK
U2 - 10.1021/la981583h
DO - 10.1021/la981583h
M3 - Article
AN - SCOPUS:0032658415
VL - 15
SP - 4434
EP - 4440
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 13
ER -
ID: 94259338