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DNA Binding with Acetate Bis(1,10-phenanthroline)silver(I) Monohydrate in a Solution and Metallization of Formed Structures. / Kasyanenko, Nina; Qiushi, Zhang; Bakulev, Vladimir; Osolodkov, Mikhail; Sokolov, Petr; Demidov, Viktor.
в: Polymers, Том 9, № 6, 211, 06.2017.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - DNA Binding with Acetate Bis(1,10-phenanthroline)silver(I) Monohydrate in a Solution and Metallization of Formed Structures
AU - Kasyanenko, Nina
AU - Qiushi, Zhang
AU - Bakulev, Vladimir
AU - Osolodkov, Mikhail
AU - Sokolov, Petr
AU - Demidov, Viktor
PY - 2017/6
Y1 - 2017/6
N2 - The study of DNA interaction with the acetate bis(1,10-phenanthroline)silver(I) monohydrate in a solution is of interest both for understanding the mechanism of biological activity of silver compound and for forming ordered structures (DNA fibrils) that can be used to solve various problems in the field of nanotechnology. The analysis of changing the DNA conformation (secondary structure, persistent length and volume effects) during the interaction by the methods of UV spectroscopy with the analysis of DNA melting, circular dichroism, viscosity, flow birefringence, AFM (atomic force microscopy) and SEM (scanning electron microscopy) was performed. The formation of two types of complexes was observed. At lower concentration of compound in DNA solution, silver atoms form the coordination bonds with a macromolecule, while the released phenanthroline ligands intercalate between DNA bases. When the concentration of the compound increases, the phenanthroline ligands form an ordered "layer" around the helix. The excess of silver compounds in the DNA solution (with more than five silver atoms per base pair), DNA precipitation is observed with the formation of long fibrils. It was shown that the binding of silver to DNA during the formation of complexes provides further metallization of the resulting structures with the aid of reducing agents; phenanthroline ligands influence the result of such metallization.
AB - The study of DNA interaction with the acetate bis(1,10-phenanthroline)silver(I) monohydrate in a solution is of interest both for understanding the mechanism of biological activity of silver compound and for forming ordered structures (DNA fibrils) that can be used to solve various problems in the field of nanotechnology. The analysis of changing the DNA conformation (secondary structure, persistent length and volume effects) during the interaction by the methods of UV spectroscopy with the analysis of DNA melting, circular dichroism, viscosity, flow birefringence, AFM (atomic force microscopy) and SEM (scanning electron microscopy) was performed. The formation of two types of complexes was observed. At lower concentration of compound in DNA solution, silver atoms form the coordination bonds with a macromolecule, while the released phenanthroline ligands intercalate between DNA bases. When the concentration of the compound increases, the phenanthroline ligands form an ordered "layer" around the helix. The excess of silver compounds in the DNA solution (with more than five silver atoms per base pair), DNA precipitation is observed with the formation of long fibrils. It was shown that the binding of silver to DNA during the formation of complexes provides further metallization of the resulting structures with the aid of reducing agents; phenanthroline ligands influence the result of such metallization.
KW - DNA
KW - bis(1,10-phenanthroline)silver(I) monohydrate
KW - DNA fibrils with silver
KW - silver reduction on DNA
KW - FLUORESCENT SILVER NANOCLUSTERS
KW - ANTICANCER ACTIVITY
KW - ANTITUMOR-ACTIVITY
KW - CRYSTAL-STRUCTURE
KW - NANOPARTICLES
KW - COMPLEXES
KW - 1,10-PHENANTHROLINE
KW - POLYMER
KW - PHENANTHROLINE
KW - DERIVATIVES
U2 - 10.3390/polym9060211
DO - 10.3390/polym9060211
M3 - статья
VL - 9
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 6
M1 - 211
ER -
ID: 9231027