DOI

  • T. Jägerbrink
  • H. Lexander
  • C. Palmberg
  • J. Shafqat
  • V. Sharoyko
  • P. O. Berggren
  • S. Efendic
  • S. Zaitsev
  • H. Jörnvall

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).

Язык оригиналаанглийский
Страницы (с-по)1310-1316
Число страниц7
ЖурналCellular and Molecular Life Sciences
Том64
Номер выпуска10
DOI
СостояниеОпубликовано - мая 2007

    Предметные области Scopus

  • Молекулярная медицина
  • Молекулярная биология
  • Фармакология
  • Клеточная и молекулрная нейробиология
  • Клеточная биология

ID: 5533079