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Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma. / Smirnov, Ilya Valerevich; Gryazeva, Irina Vladimirovna; Самойлович, Марина Платоновна; Terekhina, Lidiya Alexandrovna; Пиневич, Агния Александровна; Shashkova, Olga Alexandrovna; Krutetskaia, Irina Yurevna; Sokolov, Dmitriy Igorevich; Selkov, Sergey Alexeevich; Nikolskiy, Nikolay Nikolaevich; Klimovich, Vladimir Borisovich.

в: Immunochemistry & Immunopathology, Том 2, № 2, 30.04.2016.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Smirnov, IV, Gryazeva, IV, Самойлович, МП, Terekhina, LA, Пиневич, АА, Shashkova, OA, Krutetskaia, IY, Sokolov, DI, Selkov, SA, Nikolskiy, NN & Klimovich, VB 2016, 'Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma', Immunochemistry & Immunopathology, Том. 2, № 2.

APA

Smirnov, I. V., Gryazeva, I. V., Самойлович, М. П., Terekhina, L. A., Пиневич, А. А., Shashkova, O. A., Krutetskaia, I. Y., Sokolov, D. I., Selkov, S. A., Nikolskiy, N. N., & Klimovich, V. B. (2016). Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma. Immunochemistry & Immunopathology, 2(2).

Vancouver

Smirnov IV, Gryazeva IV, Самойлович МП, Terekhina LA, Пиневич АА, Shashkova OA и пр. Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma. Immunochemistry & Immunopathology. 2016 Апр. 30;2(2).

Author

Smirnov, Ilya Valerevich ; Gryazeva, Irina Vladimirovna ; Самойлович, Марина Платоновна ; Terekhina, Lidiya Alexandrovna ; Пиневич, Агния Александровна ; Shashkova, Olga Alexandrovna ; Krutetskaia, Irina Yurevna ; Sokolov, Dmitriy Igorevich ; Selkov, Sergey Alexeevich ; Nikolskiy, Nikolay Nikolaevich ; Klimovich, Vladimir Borisovich. / Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma. в: Immunochemistry & Immunopathology. 2016 ; Том 2, № 2.

BibTeX

@article{429648e7d5ca4da6bf1cc030708a021f,
title = "Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma",
abstract = "Soluble endoglin is produced as a result of extracellular domain cleavage of full-length membrane molecules. Numerous studies of recent years have shown that its excessive production is associated with vascular pathology, tumor growth and preeclampsia development. In this study we developed and explored ten sandwich-ELISA systems, based on monoclonal antibodies (MAbs) produced in our laboratory, to detect soluble endoglin in blood plasma. Here we demonstrated that values of endoglin concentration determined in the same samples varied greatly depending on pairs of MAbs used. Some measurements were up to two orders of magnitude higher compared with widely used commercial kit. Western blot analysis revealed four fractions of the antigen precipitated from plasma. Our data suggest that this molecular heterogeneity of soluble endoglin may lead to the pronounced differences in estimates of its concentration made using different sandwich-ELISA systems. Nevertheless, all studied systems revealed increment of endoglin content in plasma of pregnant women compared with healthy donors as well as its substantial production in preeclampsia patients.",
author = "Smirnov, {Ilya Valerevich} and Gryazeva, {Irina Vladimirovna} and Самойлович, {Марина Платоновна} and Terekhina, {Lidiya Alexandrovna} and Пиневич, {Агния Александровна} and Shashkova, {Olga Alexandrovna} and Krutetskaia, {Irina Yurevna} and Sokolov, {Dmitriy Igorevich} and Selkov, {Sergey Alexeevich} and Nikolskiy, {Nikolay Nikolaevich} and Klimovich, {Vladimir Borisovich}",
year = "2016",
month = apr,
day = "30",
language = "English",
volume = "2",
journal = "Immunochemistry & Immunopathology",
issn = "2469-9756",
publisher = "OMICS Publishing Group",
number = "2",

}

RIS

TY - JOUR

T1 - Different pairs of monoclonal antibodies detect variable amounts of soluble endoglin in human blood plasma

AU - Smirnov, Ilya Valerevich

AU - Gryazeva, Irina Vladimirovna

AU - Самойлович, Марина Платоновна

AU - Terekhina, Lidiya Alexandrovna

AU - Пиневич, Агния Александровна

AU - Shashkova, Olga Alexandrovna

AU - Krutetskaia, Irina Yurevna

AU - Sokolov, Dmitriy Igorevich

AU - Selkov, Sergey Alexeevich

AU - Nikolskiy, Nikolay Nikolaevich

AU - Klimovich, Vladimir Borisovich

PY - 2016/4/30

Y1 - 2016/4/30

N2 - Soluble endoglin is produced as a result of extracellular domain cleavage of full-length membrane molecules. Numerous studies of recent years have shown that its excessive production is associated with vascular pathology, tumor growth and preeclampsia development. In this study we developed and explored ten sandwich-ELISA systems, based on monoclonal antibodies (MAbs) produced in our laboratory, to detect soluble endoglin in blood plasma. Here we demonstrated that values of endoglin concentration determined in the same samples varied greatly depending on pairs of MAbs used. Some measurements were up to two orders of magnitude higher compared with widely used commercial kit. Western blot analysis revealed four fractions of the antigen precipitated from plasma. Our data suggest that this molecular heterogeneity of soluble endoglin may lead to the pronounced differences in estimates of its concentration made using different sandwich-ELISA systems. Nevertheless, all studied systems revealed increment of endoglin content in plasma of pregnant women compared with healthy donors as well as its substantial production in preeclampsia patients.

AB - Soluble endoglin is produced as a result of extracellular domain cleavage of full-length membrane molecules. Numerous studies of recent years have shown that its excessive production is associated with vascular pathology, tumor growth and preeclampsia development. In this study we developed and explored ten sandwich-ELISA systems, based on monoclonal antibodies (MAbs) produced in our laboratory, to detect soluble endoglin in blood plasma. Here we demonstrated that values of endoglin concentration determined in the same samples varied greatly depending on pairs of MAbs used. Some measurements were up to two orders of magnitude higher compared with widely used commercial kit. Western blot analysis revealed four fractions of the antigen precipitated from plasma. Our data suggest that this molecular heterogeneity of soluble endoglin may lead to the pronounced differences in estimates of its concentration made using different sandwich-ELISA systems. Nevertheless, all studied systems revealed increment of endoglin content in plasma of pregnant women compared with healthy donors as well as its substantial production in preeclampsia patients.

M3 - Article

VL - 2

JO - Immunochemistry & Immunopathology

JF - Immunochemistry & Immunopathology

SN - 2469-9756

IS - 2

ER -

ID: 36330639