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Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths. / Glotov, A. S.; Sinitsyna, E. S.; Danilova, M. M.; Vashukova, E. S.; Walter, J. G.; Stahl, F.; Baranov, V. S.; Vlakh, E. G.; Tennikova, T. B.

в: Talanta, Том 147, № 537-546, 15.01.2016, стр. 537-546.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Glotov, AS, Sinitsyna, ES, Danilova, MM, Vashukova, ES, Walter, JG, Stahl, F, Baranov, VS, Vlakh, EG & Tennikova, TB 2016, 'Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths', Talanta, Том. 147, № 537-546, стр. 537-546. https://doi.org/10.1016/j.talanta.2015.09.066, https://doi.org/10.1016/j.talanta.2015.09.066

APA

Glotov, A. S., Sinitsyna, E. S., Danilova, M. M., Vashukova, E. S., Walter, J. G., Stahl, F., Baranov, V. S., Vlakh, E. G., & Tennikova, T. B. (2016). Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths. Talanta, 147(537-546), 537-546. https://doi.org/10.1016/j.talanta.2015.09.066, https://doi.org/10.1016/j.talanta.2015.09.066

Vancouver

Glotov AS, Sinitsyna ES, Danilova MM, Vashukova ES, Walter JG, Stahl F и пр. Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths. Talanta. 2016 Янв. 15;147(537-546):537-546. https://doi.org/10.1016/j.talanta.2015.09.066, https://doi.org/10.1016/j.talanta.2015.09.066

Author

Glotov, A. S. ; Sinitsyna, E. S. ; Danilova, M. M. ; Vashukova, E. S. ; Walter, J. G. ; Stahl, F. ; Baranov, V. S. ; Vlakh, E. G. ; Tennikova, T. B. / Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths. в: Talanta. 2016 ; Том 147, № 537-546. стр. 537-546.

BibTeX

@article{8435b23d595140f3bfcb0e8efb3a40c2,
title = "Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths",
abstract = "Analysis of variations in DNA structure using a low-density microarray technology for routine diagnostic in evidence-based medicine is still relevant. In this work the applicability of 3-D macroporous monolithic methacrylate-based platforms for detection of different pathogenic genomic substitutions was studied. The detection of nucleotide replacements in F5 (Leiden G/A, rs6025), MTHFR (C/T, rs1801133) and ITGB3 (T/C, rs5918), involved in coagulation, and COMT (C/G, rs4818), TPH2 (T/A, rs11178997), PON1 (T/A rs854560), AGTR2 (C/A, rs11091046) and SERPINE1 (5G/4G, rs1799889), associated with pregnancy complications, was performed. The effect of such parameters as amount and type of oligonucleotide probe, amount of PCR product on signal-to-noise ratio, as well as mismatch discrimination was analyzed. Sensitivity and specificity of mutation detections were coincided and equal to 98.6%. The analysis of SERPINE1 and MTHFR genotypes by both NGS and developed microarray was performed and compared.",
keywords = "DNA microarrays, Macroporous polymer monoliths, Mutations, Pregnancy complications, Single nucleotide polymorphism (SNP)",
author = "Glotov, {A. S.} and Sinitsyna, {E. S.} and Danilova, {M. M.} and Vashukova, {E. S.} and Walter, {J. G.} and F. Stahl and Baranov, {V. S.} and Vlakh, {E. G.} and Tennikova, {T. B.}",
note = "Publisher Copyright: {\textcopyright} 2015 Elsevier B.V. All rights reserved.",
year = "2016",
month = jan,
day = "15",
doi = "10.1016/j.talanta.2015.09.066",
language = "English",
volume = "147",
pages = "537--546",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "537-546",

}

RIS

TY - JOUR

T1 - Detection of human genome mutations associated with pregnancy complications using 3-D microarray based on macroporous polymer monoliths

AU - Glotov, A. S.

AU - Sinitsyna, E. S.

AU - Danilova, M. M.

AU - Vashukova, E. S.

AU - Walter, J. G.

AU - Stahl, F.

AU - Baranov, V. S.

AU - Vlakh, E. G.

AU - Tennikova, T. B.

N1 - Publisher Copyright: © 2015 Elsevier B.V. All rights reserved.

PY - 2016/1/15

Y1 - 2016/1/15

N2 - Analysis of variations in DNA structure using a low-density microarray technology for routine diagnostic in evidence-based medicine is still relevant. In this work the applicability of 3-D macroporous monolithic methacrylate-based platforms for detection of different pathogenic genomic substitutions was studied. The detection of nucleotide replacements in F5 (Leiden G/A, rs6025), MTHFR (C/T, rs1801133) and ITGB3 (T/C, rs5918), involved in coagulation, and COMT (C/G, rs4818), TPH2 (T/A, rs11178997), PON1 (T/A rs854560), AGTR2 (C/A, rs11091046) and SERPINE1 (5G/4G, rs1799889), associated with pregnancy complications, was performed. The effect of such parameters as amount and type of oligonucleotide probe, amount of PCR product on signal-to-noise ratio, as well as mismatch discrimination was analyzed. Sensitivity and specificity of mutation detections were coincided and equal to 98.6%. The analysis of SERPINE1 and MTHFR genotypes by both NGS and developed microarray was performed and compared.

AB - Analysis of variations in DNA structure using a low-density microarray technology for routine diagnostic in evidence-based medicine is still relevant. In this work the applicability of 3-D macroporous monolithic methacrylate-based platforms for detection of different pathogenic genomic substitutions was studied. The detection of nucleotide replacements in F5 (Leiden G/A, rs6025), MTHFR (C/T, rs1801133) and ITGB3 (T/C, rs5918), involved in coagulation, and COMT (C/G, rs4818), TPH2 (T/A, rs11178997), PON1 (T/A rs854560), AGTR2 (C/A, rs11091046) and SERPINE1 (5G/4G, rs1799889), associated with pregnancy complications, was performed. The effect of such parameters as amount and type of oligonucleotide probe, amount of PCR product on signal-to-noise ratio, as well as mismatch discrimination was analyzed. Sensitivity and specificity of mutation detections were coincided and equal to 98.6%. The analysis of SERPINE1 and MTHFR genotypes by both NGS and developed microarray was performed and compared.

KW - DNA microarrays

KW - Macroporous polymer monoliths

KW - Mutations

KW - Pregnancy complications

KW - Single nucleotide polymorphism (SNP)

UR - http://www.scopus.com/inward/record.url?scp=84945431364&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2015.09.066

DO - 10.1016/j.talanta.2015.09.066

M3 - Article

C2 - 26592644

VL - 147

SP - 537

EP - 546

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 537-546

ER -

ID: 7547850