TY - JOUR

T1 - Detection of Anti-Vimentin Antibodies in Patients with Sarcoidosis

AU - Starshinova, A.

AU - Malkova, A.

AU - Zinchenko, U.

AU - Lapin, S.

AU - Mazing, A.

AU - Kudlay, D.

AU - Yablonskiy, P.

AU - Shoenfeld, Y.

N1 - Export Date: 27 February 2026; Cited By: 6; Correspondence Address: A. Starshinova; Laboratory of the Mosaic of Autoimmunity, Medical Department, St. Petersburg State University, St. Petersburg, 199034, Russian Federation; email: starshinova_777@mail.ru

PY - 2022

Y1 - 2022

N2 - There is a need to further characterize the antibody response to vimentin in relation to its possible involvement in pathogenicity of sarcoidosis and other lung disorders. Objectives: We investigated serum samples from patients with sarcoidosis, healthy controls and controls with other non-infectious lung diseases., to evaluate levels and frequency of these antibodies. Materials and methods: A retrospective-prospective comparative study was performed in the years 2015–2019. Sera from 93 patients with sarcoidosis, 55 patients with non-infectious lung diseases and 40 healthy subjects was examined for presence of autoantibodies to mutated citrullinated vimentin (anti-MCV). Patients with elevated anti-MCV levels were tested for antibodies to a cyclic citrullinated peptide (anti-CCP) and citrullinated vimentin (anti-Sa). In all cases ELISA assays was used. The results were considered statistically significant at p-value less than 0.05. Results of the study: The high concentrations of anti-MCV antibodies were more frequent in patients with sarcoidosis (40.9% of the cases, 38/93), compared to the control groups (23.6% and 25.0% of cases, respectively). In sarcoidosis, clinical symptoms similar to the autoimmune pathology were described. A moderate positive correlation between the anti-MCV and anti-Sa antibodies (r = 0.66) was found in 13 patients with sarcoidosis. There was no significant difference between the levels of the anti-MCV and the anti-CCP in patients with non-infectious lung diseases and the healthy control group. Conclusion: Antibodies to citrullinated cyclic peptides are not significant in the pathogenesis of sarcoidosis and other investigated pulmonary diseases (COPD, granulomatosis with polyangiitis, alveolitis) and based on their low concentration, it can be assumed that citrullination and modification of vimentin is not a key factor in the development of an autoimmune response in patients with sarcoidosis. © 2022 by the authors.

AB - There is a need to further characterize the antibody response to vimentin in relation to its possible involvement in pathogenicity of sarcoidosis and other lung disorders. Objectives: We investigated serum samples from patients with sarcoidosis, healthy controls and controls with other non-infectious lung diseases., to evaluate levels and frequency of these antibodies. Materials and methods: A retrospective-prospective comparative study was performed in the years 2015–2019. Sera from 93 patients with sarcoidosis, 55 patients with non-infectious lung diseases and 40 healthy subjects was examined for presence of autoantibodies to mutated citrullinated vimentin (anti-MCV). Patients with elevated anti-MCV levels were tested for antibodies to a cyclic citrullinated peptide (anti-CCP) and citrullinated vimentin (anti-Sa). In all cases ELISA assays was used. The results were considered statistically significant at p-value less than 0.05. Results of the study: The high concentrations of anti-MCV antibodies were more frequent in patients with sarcoidosis (40.9% of the cases, 38/93), compared to the control groups (23.6% and 25.0% of cases, respectively). In sarcoidosis, clinical symptoms similar to the autoimmune pathology were described. A moderate positive correlation between the anti-MCV and anti-Sa antibodies (r = 0.66) was found in 13 patients with sarcoidosis. There was no significant difference between the levels of the anti-MCV and the anti-CCP in patients with non-infectious lung diseases and the healthy control group. Conclusion: Antibodies to citrullinated cyclic peptides are not significant in the pathogenesis of sarcoidosis and other investigated pulmonary diseases (COPD, granulomatosis with polyangiitis, alveolitis) and based on their low concentration, it can be assumed that citrullination and modification of vimentin is not a key factor in the development of an autoimmune response in patients with sarcoidosis. © 2022 by the authors.

KW - autoantibodies

KW - autoimmunity

KW - granulomatous diseases

KW - mutated vimentin

KW - sarcoidosis

KW - vimentin

KW - autoantibody

KW - cyclic citrullinated peptide antibody

KW - adult

KW - antibody detection

KW - Article

KW - autoimmune disease

KW - blood sampling

KW - chronic obstructive lung disease

KW - citrullination

KW - controlled study

KW - enzyme linked immunosorbent assay

KW - female

KW - frequency

KW - human

KW - human tissue

KW - lung alveolitis

KW - lung disease

KW - lung sarcoidosis

KW - major clinical study

KW - male

KW - pathogenesis

KW - prospective study

KW - retrospective study

KW - Wegener granulomatosis

U2 - 10.3390/diagnostics12081939

DO - 10.3390/diagnostics12081939

M3 - статья

VL - 12

JO - Diagnostics

JF - Diagnostics

SN - 2075-4418

IS - 8

ER -