TY - JOUR

T1 - Description and phylogenetic relationships of Spumochlamys perforata n. sp. and Spumochlamys bryora n. sp. (Amoebozoa, Arcellinida)

AU - Kudryavtsev, Alexander

AU - Pawlowski, Jan

AU - Hausmann, Klaus

PY - 2009/11

Y1 - 2009/11

N2 - Spumochlamys perforata n. sp. and Spumochlamys bryora n. sp. were isolated and described from dry epiphytic moss. The morphology and ultrastructure of both species clearly demonstrate that they belong to the genus Spumochlamys (family Microchlamyiidae). They differ from its only described member, Spumochlamys iliensis (as well as from species of Microchlamys), in the relief of the dorsal surface of the test, revealed by scanning electron microscopy, which can represent a good characteristic for species identification. They also differ in the structure of the dorsal part of the test wall (especially S. perforata). Small subunit ribosomal DNA-based molecular phylogenetic analyses show that Spumochlamys is a deeply branching lineage of the Arcellinida, without any close affinities. Actin gene sequence analysis places this genus within the Tubulinea, close to two other arcellinid lineages but without forming a monophyletic group with them. These data together strongly suggest that the lack of resolution in the arcellinid molecular phylogenies is due to serious undersampling of taxa, a limited number of sequence data, and high divergence rates in most of the species.

AB - Spumochlamys perforata n. sp. and Spumochlamys bryora n. sp. were isolated and described from dry epiphytic moss. The morphology and ultrastructure of both species clearly demonstrate that they belong to the genus Spumochlamys (family Microchlamyiidae). They differ from its only described member, Spumochlamys iliensis (as well as from species of Microchlamys), in the relief of the dorsal surface of the test, revealed by scanning electron microscopy, which can represent a good characteristic for species identification. They also differ in the structure of the dorsal part of the test wall (especially S. perforata). Small subunit ribosomal DNA-based molecular phylogenetic analyses show that Spumochlamys is a deeply branching lineage of the Arcellinida, without any close affinities. Actin gene sequence analysis places this genus within the Tubulinea, close to two other arcellinid lineages but without forming a monophyletic group with them. These data together strongly suggest that the lack of resolution in the arcellinid molecular phylogenies is due to serious undersampling of taxa, a limited number of sequence data, and high divergence rates in most of the species.

KW - Actin

KW - Microchlamyiidae

KW - Molecular phylogeny

KW - Morphology

KW - SSU rRNA

KW - Taxonomy

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=70350519855&partnerID=8YFLogxK

U2 - 10.1111/j.1550-7408.2009.00430.x

DO - 10.1111/j.1550-7408.2009.00430.x

M3 - Article

C2 - 19883438

AN - SCOPUS:70350519855

VL - 56

SP - 495

EP - 503

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

IS - 6

ER -