TY - JOUR

T1 - Degradation of Polyribonucleotides: Biocatalysis and the Monitoring of Products

T2 - biocatalysis and the monitoring of products]

AU - Vlakh, E.G.

AU - Volokitina, M.V.

AU - Vinokhodov, D.O.

AU - Tennikova, T.B.

PY - 2014

Y1 - 2014

N2 - Macroporous monolithic material containing covalently linked ribonuclease A was used to create high-performance flow heterogeneous biocatalysts (bioreactors). The kinetic parameters of the degradation of polycytidylic acid were identified, and the properties of the obtained systems were compared. A HPLC method has been developed for monitoring products of biocatalytic degradation of RNA, and the possibility of using biocatalytic and HPLC columns in RNA degradation processes in a multicomponent mixture of biological molecules was shown.

AB - Macroporous monolithic material containing covalently linked ribonuclease A was used to create high-performance flow heterogeneous biocatalysts (bioreactors). The kinetic parameters of the degradation of polycytidylic acid were identified, and the properties of the obtained systems were compared. A HPLC method has been developed for monitoring products of biocatalytic degradation of RNA, and the possibility of using biocatalytic and HPLC columns in RNA degradation processes in a multicomponent mixture of biological molecules was shown.

UR - http://www.scopus.com/inward/record.url?scp=84927171084&partnerID=8YFLogxK

U2 - 10.7868/S0555109914060154

DO - 10.7868/S0555109914060154

M3 - Article

C2 - 25726664

VL - 50

SP - 629

EP - 636

JO - Applied Biochemistry and Microbiology

JF - Applied Biochemistry and Microbiology

SN - 0003-6838

IS - 6

ER -