TY - JOUR

T1 - Degradation of Cinnamic Acid by the Rhizospheric Strain Achromobacter insolitus LCu2

AU - Kryuchkova, E.V.

AU - Morozova, E.S.

AU - Grinev, V.S.

AU - Burygin, G.L.

AU - Gogoleva, N.E.

AU - Gogolev, Y.V.

N1 - Export Date: 21 October 2024 Адрес для корреспонденции: Kryuchkova, E.V.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Federation; эл. почта: kryu-lena@yandex.ru Сведения о финансировании: Russian Science Foundation, RSF, 22-14-00317 Текст о финансировании 1: Chromatographic analysis was carried out using the equipment of the Symbiosis Center for the Collective Use of the Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences (Sa-ratov). Текст о финансировании 2: This study was carried out within the framework of the state assignment 1022040700974-4. This work was supported by the Russian Science Foundation (complete-genome sequencing, project no. 22-14-00317).

PY - 2024/9/30

Y1 - 2024/9/30

N2 - Abstract: The strain Achromobacter insolitus LCu2 isolated from the roots of alfalfa (Medicagosativa L.) utilized cinnamic acid and its methoxy derivatives (vanillic and ferulic acids) as the sole carbon source. Weak growth was observed with m-coumaric acid, but not on o- or p-coumaric acids. Growth on the cinnamic acid was slow and diauxic. The substrate decreased in the cultivation medium by 53%; the degradation efficiency during 14 days was 30 μg/mg wet weight. Despite the bactericidal effect of cinnamic acid, the culture A. insolitus LCu2 remained viable for a long time. Genomic analysis revealed two gene clusters (hca and mhp) responsible for the dihydroxylation of the phenyl ring (hcaA1A2CDB) and its subsequent cleavage to central metabolic products (mhpACDE), as well as a transcriptional regulator (hcaR) and a putative transporter (hcaT). A putative biochemical pathway for cinnamic acid degradation by the strain A. insolitus LCu2 was predicted using genomic data. © Pleiades Publishing, Ltd. 2024. ISSN 0026-2617, Microbiology, 2024, Vol. 93, No. 5, pp. 576–584. Pleiades Publishing, Ltd., 2024.

AB - Abstract: The strain Achromobacter insolitus LCu2 isolated from the roots of alfalfa (Medicagosativa L.) utilized cinnamic acid and its methoxy derivatives (vanillic and ferulic acids) as the sole carbon source. Weak growth was observed with m-coumaric acid, but not on o- or p-coumaric acids. Growth on the cinnamic acid was slow and diauxic. The substrate decreased in the cultivation medium by 53%; the degradation efficiency during 14 days was 30 μg/mg wet weight. Despite the bactericidal effect of cinnamic acid, the culture A. insolitus LCu2 remained viable for a long time. Genomic analysis revealed two gene clusters (hca and mhp) responsible for the dihydroxylation of the phenyl ring (hcaA1A2CDB) and its subsequent cleavage to central metabolic products (mhpACDE), as well as a transcriptional regulator (hcaR) and a putative transporter (hcaT). A putative biochemical pathway for cinnamic acid degradation by the strain A. insolitus LCu2 was predicted using genomic data. © Pleiades Publishing, Ltd. 2024. ISSN 0026-2617, Microbiology, 2024, Vol. 93, No. 5, pp. 576–584. Pleiades Publishing, Ltd., 2024.

KW - Achromobacter insolitus

KW - cinnamic acid

KW - degrading bacteria

KW - dioxygenase

KW - hca

KW - mhp

UR - https://www.mendeley.com/catalogue/da2ab7de-d0e6-3ebf-a400-7dd1981a949a/

U2 - 10.1134/s0026261724604883

DO - 10.1134/s0026261724604883

M3 - статья

VL - 93

SP - 576

EP - 584

JO - МИКРОБИОЛОГИЯ

JF - МИКРОБИОЛОГИЯ

SN - 0026-3656

IS - 5

ER -