TY - JOUR

T1 - α-Defensins from blood leukocytes of the monkey Papio hamadryas

AU - Tsvetkova, E. V.

AU - Aleshina, G. M.

AU - Shamova, O. V.

AU - Leonova, L. E.

AU - Lehrer, R. I.

AU - Kokryakov, V. N.

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Three antimicrobial peptides named PHD1-3 (Papio hamadryas defensin) have been isolated from hamadryas baboon blood leukocytes using preparative electrophoresis and reverse-phase HPLC. The primary structures of these peptides have been determined by automated Edman degradation and mass-spectrometry. The results suggest that the peptides belong to the α-defensin family. Structural homology analysis reveals that among α-defensins from other animal species, PHD3 is the most closely related to RMAD5 (rhesus macaque α-defensin) (90% homology) from rhesus macaque leukocytes and also highly similar to human α-defensin HD5 (60% homology), which is produced by intestinal Paneth cells. The homology of PHD3 with human neutrophil α-defensin HNP1 (human natural peptide) was 30%. The primary structures of PHD1 and PHD2 are most similar to RED1 (rhesus enteral defensin), one of six enteral α-defensins of rhesus monkeys. PHD1-3 have been shown to be active against the Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus, the Gram-negative bacterium Escherichia coli, and the fungus Candida albicans, similarly to the human HNP1 defensin.

AB - Three antimicrobial peptides named PHD1-3 (Papio hamadryas defensin) have been isolated from hamadryas baboon blood leukocytes using preparative electrophoresis and reverse-phase HPLC. The primary structures of these peptides have been determined by automated Edman degradation and mass-spectrometry. The results suggest that the peptides belong to the α-defensin family. Structural homology analysis reveals that among α-defensins from other animal species, PHD3 is the most closely related to RMAD5 (rhesus macaque α-defensin) (90% homology) from rhesus macaque leukocytes and also highly similar to human α-defensin HD5 (60% homology), which is produced by intestinal Paneth cells. The homology of PHD3 with human neutrophil α-defensin HNP1 (human natural peptide) was 30%. The primary structures of PHD1 and PHD2 are most similar to RED1 (rhesus enteral defensin), one of six enteral α-defensins of rhesus monkeys. PHD1-3 have been shown to be active against the Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus, the Gram-negative bacterium Escherichia coli, and the fungus Candida albicans, similarly to the human HNP1 defensin.

KW - α-defensins

KW - Antimicrobial peptides of mammals

KW - Leukocytes

KW - Monkey

KW - Papio hamadryas

KW - Primary structure

UR - http://www.scopus.com/inward/record.url?scp=33747690989&partnerID=8YFLogxK

U2 - 10.1134/S0006297906080098

DO - 10.1134/S0006297906080098

M3 - Article

C2 - 16978151

AN - SCOPUS:33747690989

VL - 71

SP - 879

EP - 883

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - 8

ER -