Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still's Disease. / Myachikova, Valentina; Kudryavtsev, Igor; Rubinstein, Artem; Aquino, Arthur; Isakov, Dmitry; Golovkin, Alexey; Maslyanskiy, Alexey.
в: Current Issues in Molecular Biology, Том 46, № 2, 01.02.2024, стр. 1177-1191.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still's Disease
AU - Myachikova, Valentina
AU - Kudryavtsev, Igor
AU - Rubinstein, Artem
AU - Aquino, Arthur
AU - Isakov, Dmitry
AU - Golovkin, Alexey
AU - Maslyanskiy, Alexey
PY - 2024/2/1
Y1 - 2024/2/1
N2 - Adult-onset Still's disease (AOSD) is a complex systemic inflammatory disorder, categorized as an 'IL-1 driven' inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- and B-cell subsets. T-cell and B-cell subsets were significantly altered in patients with AOSD. Within CD4+ T cells, Th2 cells were decreased. Additionally, Th17 cell and follicular Th cell subsets were altered within CD45RA-CD62L+ and CD45RA-CD62L- Th cells in patients with AOSD compared to healthy controls. We identified changes in CD8+ T cell maturation and 'polarization' in AOSD patients, with an elevated presence of the TEMRA CD8+ T cell subset. Furthermore, the percentage of Tc1 cells was decreased, while the frequency of CCR6-CXCR3- Tc2 cells was elevated. Finally, we determined that the frequency of CD5+CD27- B cells was dramatically decreased in patients with AOSD compared to healthy controls. Further investigations on a large group of patients with AOSD are required to evaluate these adaptive immunity cells in the disease pathogenesis.
AB - Adult-onset Still's disease (AOSD) is a complex systemic inflammatory disorder, categorized as an 'IL-1 driven' inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- and B-cell subsets. T-cell and B-cell subsets were significantly altered in patients with AOSD. Within CD4+ T cells, Th2 cells were decreased. Additionally, Th17 cell and follicular Th cell subsets were altered within CD45RA-CD62L+ and CD45RA-CD62L- Th cells in patients with AOSD compared to healthy controls. We identified changes in CD8+ T cell maturation and 'polarization' in AOSD patients, with an elevated presence of the TEMRA CD8+ T cell subset. Furthermore, the percentage of Tc1 cells was decreased, while the frequency of CCR6-CXCR3- Tc2 cells was elevated. Finally, we determined that the frequency of CD5+CD27- B cells was dramatically decreased in patients with AOSD compared to healthy controls. Further investigations on a large group of patients with AOSD are required to evaluate these adaptive immunity cells in the disease pathogenesis.
U2 - 10.3390/cimb46020075
DO - 10.3390/cimb46020075
M3 - Article
C2 - 38392193
VL - 46
SP - 1177
EP - 1191
JO - Current Issues in Molecular Biology
JF - Current Issues in Molecular Biology
SN - 1467-3037
IS - 2
ER -
ID: 117429786