DOI

  • Kira Vyatkina
  • Lennard J.M. Dekker
  • Si Wu
  • Martijn M. VanDuijn
  • Xiaowen Liu
  • Nikola Tolić
  • Theo M. Luider
  • Ljiljana Paša-Tolić

Despite high-resolution mass spectrometers are becoming accessible for more and more laboratories, tandem (MS/MS) mass spectra are still often collected at a low resolution. And even if acquired at a high resolution, software tools used for their processing do not tend to benefit from that in full, and an ability to specify a relative mass tolerance in this case often remains the only feature the respective algorithms take advantage of. We argue that a more efficient way to analyze high-resolution MS/MS spectra should be with methods more explicitly accounting for the precision level, and sustain this claim through demonstrating that a de novo sequencing framework originally developed for (high-resolution) top-down MS/MS data is perfectly suitable for processing high-resolution bottom-up datasets, even though a top-down like deconvolution performed as the first step will leave in many spectra at most a few peaks.

Язык оригиналаанглийский
Номер статьи1600321
Число страниц9
ЖурналProteomics
Том17
Номер выпуска23-24
Дата раннего онлайн-доступа7 ноя 2017
DOI
СостояниеОпубликовано - дек 2017

    Предметные области Scopus

  • Молекулярная биология
  • Биохимия

ID: 27002527