Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Despite high-resolution mass spectrometers are becoming accessible for more and more laboratories, tandem (MS/MS) mass spectra are still often collected at a low resolution. And even if acquired at a high resolution, software tools used for their processing do not tend to benefit from that in full, and an ability to specify a relative mass tolerance in this case often remains the only feature the respective algorithms take advantage of. We argue that a more efficient way to analyze high-resolution MS/MS spectra should be with methods more explicitly accounting for the precision level, and sustain this claim through demonstrating that a de novo sequencing framework originally developed for (high-resolution) top-down MS/MS data is perfectly suitable for processing high-resolution bottom-up datasets, even though a top-down like deconvolution performed as the first step will leave in many spectra at most a few peaks.
Язык оригинала | английский |
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Номер статьи | 1600321 |
Число страниц | 9 |
Журнал | Proteomics |
Том | 17 |
Номер выпуска | 23-24 |
Дата раннего онлайн-доступа | 7 ноя 2017 |
DOI | |
Состояние | Опубликовано - дек 2017 |
ID: 27002527