Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Control of arginine biosynthesis in the green alga Dunaliella salina. / Лапина, Татьяна Викторовна; Ермилова, Елена Викторовна; Власова, Виталина Анатольевна; Залуцкая, Жаннета Михайловна; Пузанский, Роман Константинович; Аношкина, Лидия Дмитриевна.
в: Protistology, Том 18, № 3, 09.2024, стр. 212-220.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Control of arginine biosynthesis in the green alga Dunaliella salina
AU - Лапина, Татьяна Викторовна
AU - Ермилова, Елена Викторовна
AU - Власова, Виталина Анатольевна
AU - Залуцкая, Жаннета Михайловна
AU - Пузанский, Роман Константинович
AU - Аношкина, Лидия Дмитриевна
PY - 2024/9
Y1 - 2024/9
N2 - Until now, the question of how arginine (Arg) biosynthesis operates in oxygenicphototrophs at high salt concentrations has not been addressed experimentally.This makes a green alga Dunaliella salina attractive for studying the molecularevents underlying the adjustment of Arg metabolism to high salinity. Enzyme assayand Western blotting show that N-acetyl-L-glutamate kinase (NAGK) activitydecreases with increasing salinity and DsPII expression in cells is impaired.Using GC-MS analysis, we also identified that the resulting levels of free Argare dependent on ornithine flux to putrescine and proline. These observationssuggest that NAGKs may have an alternative common PII-independent modeof activation, raising important questions about the relationship between NAGKcontrol and the development of salinity tolerance during evolution.
AB - Until now, the question of how arginine (Arg) biosynthesis operates in oxygenicphototrophs at high salt concentrations has not been addressed experimentally.This makes a green alga Dunaliella salina attractive for studying the molecularevents underlying the adjustment of Arg metabolism to high salinity. Enzyme assayand Western blotting show that N-acetyl-L-glutamate kinase (NAGK) activitydecreases with increasing salinity and DsPII expression in cells is impaired.Using GC-MS analysis, we also identified that the resulting levels of free Argare dependent on ornithine flux to putrescine and proline. These observationssuggest that NAGKs may have an alternative common PII-independent modeof activation, raising important questions about the relationship between NAGKcontrol and the development of salinity tolerance during evolution.
KW - arginine, Dunaliella salina, N-acetyl-L-glutamate kinase, salinity tolerance
U2 - 10.21685/1680-0826-2024-18-3-3
DO - 10.21685/1680-0826-2024-18-3-3
M3 - Article
VL - 18
SP - 212
EP - 220
JO - Protistology
JF - Protistology
SN - 1680-0826
IS - 3
ER -
ID: 124615800