TY - JOUR

T1 - Connecting the Dots: Does COI Variability in Paramecium caudatum (Ciliates, Protista) Support the Moderate Endemicity Model of Microeukaryote Distribution?

AU - Tarcz, S

AU - Krenek, S

AU - Berendonk, TU

AU - Rautian, M

AU - Surmacz, M

AU - Przybos, E

N1 - Times Cited in Web of Science Core Collection: 0 Total Times Cited: 0 Cited Reference Count: 129

PY - 2026/4/1

Y1 - 2026/4/1

N2 - Aim Understanding the biodiversity and distribution patterns of microbial eukaryotes is fundamental to biosphere research. The biogeography of free-living protists remains contentious, with the 'everything is everywhere' and 'moderate endemicity' hypotheses representing competing paradigms. Here, we investigate whether and how global genetic variability within the cosmopolitan species Paramecium caudatum conforms to these models. We further examine whether genetic diversity within the COI gene can indicate the presence of cryptic species.Location Global study encompassing samples from nearly all major biogeographic realms, including the Palearctic, Nearctic, Neotropical, Indomalayan, and Australasian regions.Time Period Synthetic analysis combining historical data from public databases (GenBank) with newly collected data from strains isolated between 2015 and 2020.Major Taxa Studied The ciliate Paramecium caudatum.Methods We analysed the mitochondrial cytochrome c oxidase subunit I (COI) gene fragment from over 300 strains of P. caudatum, combining 231 newly sequenced samples with 103 sequences retrieved from the GenBank database. Phylogenetic relationships were reconstructed using maximum likelihood (ML), maximum parsimony (MP), neighbour-joining (NJ), and Bayesian inference (BI) methods. Haplotype networks were constructed using the median-joining method to assess genetic relationships and biogeographical patterns.Results Analysis revealed five distinct COI haplogroups (A, B, C, D and E) within a P. caudatum clade, each exhibiting distinct biogeographical signatures. Two haplogroups (A and B) displayed broad intercontinental distributions. In contrast, three others (C, D and E) showed restricted or endemic ranges, being confined to the Nearctic, northern Western Palearctic, and Indomalayan realms, respectively. The study revealed substantial intraspecific variability (Hd = 0.9252). Neutrality tests (Fu's Fs and Tajima's D) and congruence with previously published nuclear genomic data indicate that the major identified haplogroups are not random clusters but represent expanding evolutionary lineages.Main Conclusions Our findings demonstrate that while P. caudatum exhibits cosmopolitan distribution as a morphospecies, its intraspecific genetic structure displays pronounced heterogeneity at the global scale. The co-occurrence of haplogroups with contrasting distribution patterns, both widespread and endemic, provides compelling support for the moderate endemicity model in microbial eukaryote biogeography. These results underscore the existence of cryptic biodiversity and emphasise the need for high-resolution genetic analyses to gain a comprehensive understanding of microbial ecology and evolutionary processes.

AB - Aim Understanding the biodiversity and distribution patterns of microbial eukaryotes is fundamental to biosphere research. The biogeography of free-living protists remains contentious, with the 'everything is everywhere' and 'moderate endemicity' hypotheses representing competing paradigms. Here, we investigate whether and how global genetic variability within the cosmopolitan species Paramecium caudatum conforms to these models. We further examine whether genetic diversity within the COI gene can indicate the presence of cryptic species.Location Global study encompassing samples from nearly all major biogeographic realms, including the Palearctic, Nearctic, Neotropical, Indomalayan, and Australasian regions.Time Period Synthetic analysis combining historical data from public databases (GenBank) with newly collected data from strains isolated between 2015 and 2020.Major Taxa Studied The ciliate Paramecium caudatum.Methods We analysed the mitochondrial cytochrome c oxidase subunit I (COI) gene fragment from over 300 strains of P. caudatum, combining 231 newly sequenced samples with 103 sequences retrieved from the GenBank database. Phylogenetic relationships were reconstructed using maximum likelihood (ML), maximum parsimony (MP), neighbour-joining (NJ), and Bayesian inference (BI) methods. Haplotype networks were constructed using the median-joining method to assess genetic relationships and biogeographical patterns.Results Analysis revealed five distinct COI haplogroups (A, B, C, D and E) within a P. caudatum clade, each exhibiting distinct biogeographical signatures. Two haplogroups (A and B) displayed broad intercontinental distributions. In contrast, three others (C, D and E) showed restricted or endemic ranges, being confined to the Nearctic, northern Western Palearctic, and Indomalayan realms, respectively. The study revealed substantial intraspecific variability (Hd = 0.9252). Neutrality tests (Fu's Fs and Tajima's D) and congruence with previously published nuclear genomic data indicate that the major identified haplogroups are not random clusters but represent expanding evolutionary lineages.Main Conclusions Our findings demonstrate that while P. caudatum exhibits cosmopolitan distribution as a morphospecies, its intraspecific genetic structure displays pronounced heterogeneity at the global scale. The co-occurrence of haplogroups with contrasting distribution patterns, both widespread and endemic, provides compelling support for the moderate endemicity model in microbial eukaryote biogeography. These results underscore the existence of cryptic biodiversity and emphasise the need for high-resolution genetic analyses to gain a comprehensive understanding of microbial ecology and evolutionary processes.

KW - biodiversity

KW - cryptic species

KW - haplogroups

KW - model microeukaryote

KW - protist biogeography

KW - species discrimination

KW - AURELIA COMPLEX

KW - MITOCHONDRIAL-DNA

KW - SPECIES COMPOSITION

KW - GENETIC DIVERSITY

KW - CILIOPHORA

KW - EXISTENCE

KW - EVOLUTION

KW - ECOLOGY

KW - OLIGOHYMENOPHOREA

KW - IDENTIFICATION

UR - https://www.mendeley.com/catalogue/1e5fc5c0-dc24-37d7-8a17-431250ec8113/

U2 - 10.1111/jbi.70206

DO - 10.1111/jbi.70206

M3 - статья

VL - 53

JO - Journal of Biogeography

JF - Journal of Biogeography

SN - 0305-0270

IS - 4

ER -