TY - JOUR

T1 - Complete genome sequence of polynucleobacter necessarius subsp. Asymbioticus type strain (QLW-P1DMWA-1 T)

AU - Meincke, Linda

AU - Copeland, Alex

AU - Lapidus, Alla

AU - Lucas, Susan

AU - Berry, Kerrie W.

AU - del Rio, Tijana Glavina

AU - Hammon, Nancy

AU - Dalin, Eileen

AU - Tice, Hope

AU - Pitluck, Sam

AU - Richardson, Paul

AU - Bruce, David

AU - Goodwin, Lynne

AU - Han, Cliff

AU - Tapia, Roxanne

AU - Detter, John C.

AU - Schmutz, Jeremy

AU - Brettin, Thomas

AU - Larimer, Frank

AU - Land, Miriam

AU - Hauser, Loren

AU - Kyrpides, Nikos C.

AU - Ivanova, Natalia

AU - Göker, Markus

AU - Woyke, Tanja

AU - Wu, Qinglong L.

AU - Pöckl, Matthias

AU - Hahn, Martin W.

AU - Klenk, Hans Peter

PY - 2012/4/2

Y1 - 2012/4/2

N2 - Polynucleobacter necessarius subsp. asymbioticus strain QLW-P1DMWA-1 T is a planktonic freshwater bacterium affiliated with the family Burkholderiaceae (class Betaproteobacteria). This strain is of interest because it represents a subspecies with cosmopolitan and ubiquitous distribution in standing freshwater systems. The 16S-23S ITS genotype represented by the se-quenced strain comprised on average more than 10% of bacterioplankton in its home habitat. While all strains of the subspecies P. necessarius asymbioticus are free-living freshwater bacteria, strains belonging to the only other subspecies, P. necessarius subsp. necessarius are ob-ligate endosymbionts of the ciliate Euplotes aediculatus. The two subspecies of P. necessarius are the instances of two closely related subspecies that differ in their lifestyle (free-living vs. obligate endosymbiont), and they are the only members of the genus Polynucleobacter with completely sequenced genomes. Here we describe the features of P. necessarius subsp. asymbioticus, together with the complete genome sequence and annotation. The 2,159,490 bp long chromosome with a total of 2,088 protein-coding and 48 RNA genes is the first com-pleted genome sequence of the genus Polynucleobacter to be published and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2006.

AB - Polynucleobacter necessarius subsp. asymbioticus strain QLW-P1DMWA-1 T is a planktonic freshwater bacterium affiliated with the family Burkholderiaceae (class Betaproteobacteria). This strain is of interest because it represents a subspecies with cosmopolitan and ubiquitous distribution in standing freshwater systems. The 16S-23S ITS genotype represented by the se-quenced strain comprised on average more than 10% of bacterioplankton in its home habitat. While all strains of the subspecies P. necessarius asymbioticus are free-living freshwater bacteria, strains belonging to the only other subspecies, P. necessarius subsp. necessarius are ob-ligate endosymbionts of the ciliate Euplotes aediculatus. The two subspecies of P. necessarius are the instances of two closely related subspecies that differ in their lifestyle (free-living vs. obligate endosymbiont), and they are the only members of the genus Polynucleobacter with completely sequenced genomes. Here we describe the features of P. necessarius subsp. asymbioticus, together with the complete genome sequence and annotation. The 2,159,490 bp long chromosome with a total of 2,088 protein-coding and 48 RNA genes is the first com-pleted genome sequence of the genus Polynucleobacter to be published and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2006.

KW - Aerobic

KW - Betaproteobacteria

KW - Burkholderiaceae

KW - Chemoorganotrophic

KW - CSP2006

KW - Free-living

KW - Freshwater habitats

KW - Gram-negative

KW - Non-motile

KW - Planktonic

UR - http://www.scopus.com/inward/record.url?scp=84858697506&partnerID=8YFLogxK

U2 - 10.4056/sigs.2395367

DO - 10.4056/sigs.2395367

M3 - Article

AN - SCOPUS:84858697506

VL - 6

SP - 74

EP - 83

JO - Standards in Genomic Sciences

JF - Standards in Genomic Sciences

SN - 1944-3277

IS - 1

ER -