DOI

The tonoplast is an intracellular membrane, important for numerous functions of the vacuoles in plant cells. Transport, signaling, enzymatic activity and other processes of the tonoplast are the focus of a wide spectrum of studies. Multiple advanced analyses demand highly purified vacuoles or vesicles of the tonoplast membrane fraction. Since 1960 several approaches have been developed for such purification, but new goals and new model objects require adjustment of already existing techniques. The presented investigation aimed to compare two methods of tonoplast membrane fraction purification from tobacco suspension cell culture BY-2 (Nicotiana tabacum L., cv Bright Yellow) based on sucrose and sucrose/sorbitol gradients. The functional activity of obtained purified tonoplast fractions was measured by the hydrolytic activity of vacuolar H+-ATPase and H+-PPase.

Язык оригиналаанглийский
Страницы (с-по)178-186
Число страниц9
ЖурналBiological Communications
Том65
Номер выпуска2
DOI
СостояниеОпубликовано - июн 2020

    Предметные области Scopus

  • Биохимия, генетика и молекулярная биология (все)
  • Земледелие и биологические науки (все)

ID: 71519156