Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The tonoplast is an intracellular membrane, important for numerous functions of the vacuoles in plant cells. Transport, signaling, enzymatic activity and other processes of the tonoplast are the focus of a wide spectrum of studies. Multiple advanced analyses demand highly purified vacuoles or vesicles of the tonoplast membrane fraction. Since 1960 several approaches have been developed for such purification, but new goals and new model objects require adjustment of already existing techniques. The presented investigation aimed to compare two methods of tonoplast membrane fraction purification from tobacco suspension cell culture BY-2 (Nicotiana tabacum L., cv Bright Yellow) based on sucrose and sucrose/sorbitol gradients. The functional activity of obtained purified tonoplast fractions was measured by the hydrolytic activity of vacuolar H+-ATPase and H+-PPase.
Язык оригинала | английский |
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Страницы (с-по) | 178-186 |
Число страниц | 9 |
Журнал | Biological Communications |
Том | 65 |
Номер выпуска | 2 |
DOI | |
Состояние | Опубликовано - июн 2020 |
ID: 71519156